Abstract
The guanine plus cytosine (GC) content of the deoxyribonucleic acid (DNA) of 11 isolates of host-dependent (H-D) bdellovibrios and 18 host-independent (H-I) derivatives was determined from thermal denaturation curves and buoyant densities in CsCl. The H-D and respective H-I cultures have GC contents which are identical within the limits of experimental error. Most cultures of Bdellovibrio bacteriovorus, including the holotype culture, have 50.4 ± 0.9 moles% GC in their DNA; two bdellovibrio isolates of presently uncertain nomenclatural status contain DNA of about 43% GC. Optical melting profiles of all the DNA from all of these organisms are particularly steep, indicating little compositional heterogeneity. Chromatography of acid hydrolysates of Bdellovibrio nucleic acids reveal no unusual components. The DNA content per cell of one H-I derivative is about one-third the amount per Escherichia coli cell growing at a comparable rate.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DOTY P., MARMUR J., SUEOKA N. The heterogeneity in properties and functioning of deoxyribonucleic acids. Brookhaven Symp Biol. 1959 Nov;12:1–16. [PubMed] [Google Scholar]
- Lark C. Regulation of deoxyribonucleic acid synthesis in Escherichia coli: dependence on growth rates. Biochim Biophys Acta. 1966 Jun 22;119(3):517–525. doi: 10.1016/0005-2787(66)90128-6. [DOI] [PubMed] [Google Scholar]
- MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962 Jul;5:109–118. doi: 10.1016/s0022-2836(62)80066-7. [DOI] [PubMed] [Google Scholar]
- MARMUR J., DOTY P. Heterogeneity in deoxyribonucleic acids. I. Dependence on composition of the configurational stability of deoxyribonucleic acids. Nature. 1959 May 23;183(4673):1427–1429. doi: 10.1038/1831427a0. [DOI] [PubMed] [Google Scholar]
- Mandel M. Deoxyribonucleic acid base composition in the genus Pseudomonas. J Gen Microbiol. 1966 May;43(2):273–292. doi: 10.1099/00221287-43-2-273. [DOI] [PubMed] [Google Scholar]
- SCHAECHTER M., MAALOE O., KJELDGAARD N. O. Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):592–606. doi: 10.1099/00221287-19-3-592. [DOI] [PubMed] [Google Scholar]
- SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
- STOLP H., STARR M. P. BDELLOVIBRIO BACTERIOVORUS GEN. ET SP. N., A PREDATORY, ECTOPARASITIC, AND BACTERIOLYTIC MICROORGANISM. Antonie Van Leeuwenhoek. 1963;29:217–248. doi: 10.1007/BF02046064. [DOI] [PubMed] [Google Scholar]
- Seidler R. J., Starr M. P. Isolation and characterization of host-independent Bdellovibrios. J Bacteriol. 1969 Nov;100(2):769–785. doi: 10.1128/jb.100.2.769-785.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]