Abstract
A chromosomal lesion responsible for defective potassium ion uptake in Escherichia coli B has been mapped by use of standard interrupted-mating crosses. The mutation, kac-1, is in strain RD-2, which is deficient in K+ intake, exchanges cell K+ for extracellular isotope at a reduced rate, and has an abnormality of phosphorus metabolism associated with its potassium deficiency. This report places kac-1 at about 4 min clockwise from pro, close to gal. The locus of kac-1 is distinctly different from the potassium retention mutant in strain B-207, the only other potassium accumulation mutant mapped in E. coli so far. In this study, two other potassium accumulation mutations, kac-2 and kac-3, whose particular type of accumulation defects have not yet been determined, were mapped. These mutations are in the same region of the chromosome as kac-1.
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Selected References
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- BOYER H. GENETIC CONTROL OF RESTRICTION AND MODIFICATION IN ESCHERICHIA COLI. J Bacteriol. 1964 Dec;88:1652–1660. doi: 10.1128/jb.88.6.1652-1660.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boyer H. Conjugation in Escherichia coli. J Bacteriol. 1966 May;91(5):1767–1772. doi: 10.1128/jb.91.5.1767-1772.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Copeland J. C., Bryson V. Restriction in matings of Escherichia coli strain K-12 with strain B. Genetics. 1966 Aug;54(2):441–452. doi: 10.1093/genetics/54.2.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DAMADIAN R., SOLOMON A. K. BACTERIAL MUTANT WITH IMPAIRED POTASSIUM TRANSPORT AND METHIONINE BIOSYNTHESIS. Science. 1964 Sep 18;145(3638):1327–1328. doi: 10.1126/science.145.3638.1327. [DOI] [PubMed] [Google Scholar]
- DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Damadian R. Abnormal phosphorus metabolism in a potassium transport mutant of Escherichia coli. Biochim Biophys Acta. 1967 May 2;135(2):378–380. doi: 10.1016/0005-2736(67)90137-x. [DOI] [PubMed] [Google Scholar]
- Damadian R. Ion metabolism in a potassium accumulation mutant of Escherichia coli B. I. Potassium metabolism. J Bacteriol. 1968 Jan;95(1):113–122. doi: 10.1128/jb.95.1.113-122.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harold F. M., Harold R. L., Baarda J. R., Abrams A. A genetic defect in retention of potassium by Streptococcus faecalis. Biochemistry. 1967 Jun;6(6):1777–1784. doi: 10.1021/bi00858a028. [DOI] [PubMed] [Google Scholar]
- LUBIN M. Enrichment of auxotrophic mutant populations by recycling. J Bacteriol. 1962 Mar;83:696–697. doi: 10.1128/jb.83.3.696-697.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LUBOCHINSKY B., MEURY J., STOLKOWSKI J. CIN'ETIQUE DES 'ECHANGES DE POTASSIUM CHEZ L'ESCHERICHIA COLI, SOUCHE B 207, QUI NE PEUT CRO ITRE NORMALEMENT QU'EN PR'ESENCE DE CONCENTRATIONS 'ELEV'EES EN POTASSIUM. C R Hebd Seances Acad Sci. 1964 May 20;258:5106–5109. [PubMed] [Google Scholar]
- LURIA S. E., BURROUS J. W. Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957 Oct;74(4):461–476. doi: 10.1128/jb.74.4.461-476.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lubochinsky B., Meury J., Stolkowski J. Transport du potassium et synthèse des phospholipides chez l'Escherichia coli. II. Cinétiques comparées de l'incorporation de 32P phosphate dans les phospholipides et du transport simultané de K+ dans les souches B163 et B525. Bull Soc Chim Biol (Paris) 1966;48(7):873–885. [PubMed] [Google Scholar]
- Slayman C. W., Tatum E. L. Potassium transport in Neurospora. 3. Isolation of a transport mutant. Biochim Biophys Acta. 1965 Sep 27;109(1):184–193. doi: 10.1016/0926-6585(65)90102-0. [DOI] [PubMed] [Google Scholar]
- Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]