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. 1969 Nov;100(2):890–894. doi: 10.1128/jb.100.2.890-894.1969

Reductive Pentose Cycle and Formate Assimilation in Rhodopseudomonas palustris

Jean E Stokes 1, Derek S Hoare 1
PMCID: PMC250172  PMID: 5354954

Abstract

Rhodopseudomonas palustris assimilated formate autotrophically as carbon dioxide and hydrogen arising from the activity of the formic hydrogenlyase system. Kinetic analyses of cell suspensions pulse-labeled with 14C-formate or 14C-bicarbonate showed similar distributions of incorporated radioactivity. In both cases phosphate esters were the first assimilation products. Ribulose diphosphate carboxylase, phosphoribose isomerase, and phosphoribulokinase, characteristic enzymes of the reductive pentose cycle, were present in extracts of cells grown on formate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson L., Fuller R. C. Photosynthesis in Rhodospirillum rubrum. I. Autotrophic carbon dioxide fixation. Plant Physiol. 1967 Apr;42(4):487–490. doi: 10.1104/pp.42.4.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BANDURSKI R. S., AXELROD B. The chromatographic identification of some biologically important phosphate esters. J Biol Chem. 1951 Nov;193(1):405–410. [PubMed] [Google Scholar]
  3. Evans M. C., Buchanan B. B., Arnon D. I. A new ferredoxin-dependent carbon reduction cycle in a photosynthetic bacterium. Proc Natl Acad Sci U S A. 1966 Apr;55(4):928–934. doi: 10.1073/pnas.55.4.928. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FULLER R. C., SMILLIE R. M., SISLER E. C., KORNBERG H. L. Carbon metabolism in Chromatium. J Biol Chem. 1961 Jul;236:2140–2149. [PubMed] [Google Scholar]
  5. GLOVER J., KAMEN M. D., VAN GENDEREN H. Studies on the metabolism of photosynthetic bacteria. XII. Comparative light and dark metabolism of acetate and carbonate by Rhodospirillum rubrum. Arch Biochem Biophys. 1952 Feb;35(2):384–408. doi: 10.1016/s0003-9861(52)80020-7. [DOI] [PubMed] [Google Scholar]
  6. HOARE D. S. The photo-assimilation of acetate by Rhodospirillum rubrum. Biochem J. 1963 May;87:284–301. doi: 10.1042/bj0870284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HURLBERT R. E., LASCELLES J. RIBULOSE DIPHOSPHATE CARBOXYLASE IN THIORHODACEAE. J Gen Microbiol. 1963 Dec;33:445–458. doi: 10.1099/00221287-33-3-445. [DOI] [PubMed] [Google Scholar]
  8. KNIGHT M. The photometabolism of propionate by Rhodospirillum rubrum. Biochem J. 1962 Jul;84:170–185. doi: 10.1042/bj0840170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KORNBERG H. L., LASCELLES J. The formation of isocitratase by the Athiorhodaceae. J Gen Microbiol. 1960 Dec;23:511–517. doi: 10.1099/00221287-23-3-511. [DOI] [PubMed] [Google Scholar]
  10. Klemme J. H. Untersuchungen zur Photoautotrophie mit molekularem Wasserstoff bei neuisolierten schwefelfreien Purpurbakterien. Arch Mikrobiol. 1968;64(1):29–42. [PubMed] [Google Scholar]
  11. LARGE P. J., PEEL D., QUAYLE J. R. Microbial growth on C1 compounds. II. Synthesis of cell constituents by methanol- and formate-grown Pseudomonas AM 1, and methanol-grown Hyphomicrobium vulgare. Biochem J. 1961 Dec;81:470–480. doi: 10.1042/bj0810470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Large P. J., Quayle J. R. Microbial growth on C(1) compounds. 5. Enzyme activities in extracts of Pseudomonas AM1. Biochem J. 1963 May;87(2):386–396. doi: 10.1042/bj0870386. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. London J., Rittenberg S. C. Effects of organic matter on the growth of Thiobacillus intermedius. J Bacteriol. 1966 Mar;91(3):1062–1069. doi: 10.1128/jb.91.3.1062-1069.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. ORMEROD J. G. The use of radioactive carbon dioxide in the measurement of carbon dioxide fixation in Rhodospirillum rubrum. Biochem J. 1956 Oct;64(2):373–380. doi: 10.1042/bj0640373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. QUAYLE J. R., KEECH D. B. Carbon assimilation by Pseudomonas oxalaticus (OX 1). 1. Formate and carbon dioxide utilization during growth on formate. Biochem J. 1959 Aug;72:623–630. doi: 10.1042/bj0720623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Qadri S. M., Hoare D. S. Formic hydrogenlyase and the photoassimilation of formate by a strain of Rhodopseudomonas palustris. J Bacteriol. 1968 Jun;95(6):2344–2357. doi: 10.1128/jb.95.6.2344-2357.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. RYLE A. P., SANGER F., SMITH L. F., KITAI R. The disulphide bonds of insulin. Biochem J. 1955 Aug;60(4):541–556. doi: 10.1042/bj0600541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. STOPPANI A. O., FULLER R. C., CALVIN M. Carbon dioxide fixation by Rhodopseudomonas capsulatus. J Bacteriol. 1955 May;69(5):491–501. doi: 10.1128/jb.69.5.491-501.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Smith A. J., Hoare D. S. Acetate assimilation by Nitrobacter agilis in relation to its "obligate autotrophy". J Bacteriol. 1968 Mar;95(3):844–855. doi: 10.1128/jb.95.3.844-855.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. VANDERHEIDEN B. S. SEPARATION, IDENTIFICATION, AND QUANTITATIVE DETERMINATION OF P 32-LABELED PHOSPHATE ESTERS FROM ERYTHROCYTES. Anal Biochem. 1964 May;8:1–19. doi: 10.1016/0003-2697(64)90163-0. [DOI] [PubMed] [Google Scholar]
  21. Yoch D. C., Lindstrom E. S. Photosynthetic conversion of formate and CO2 to glutamate by rhodopseudomonas palustris. Biochem Biophys Res Commun. 1967 Jul 10;28(1):65–69. doi: 10.1016/0006-291x(67)90407-x. [DOI] [PubMed] [Google Scholar]

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