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. 1969 Nov;100(2):1049–1061. doi: 10.1128/jb.100.2.1049-1061.1969

Comparative Study of the Structure of Gas Vacuoles

Germaine Cohen-Bazire 1,2, Riyo Kunisawa 1,2, Norbert Pfennig 1,2
PMCID: PMC250193  PMID: 4982667

Abstract

The fine structure of gas vacuoles was examined in two blue-green algae, two green bacteria, three purple sulfur bacteria, and two halobacteria. The gas vacuole is a compound organelle, composed of a variable number of gas vesicles. These are closed, cylindrical, gas-containing structures with conical ends, about 80 to 100 nm in width and of variable length, ranging from 0.2 to over 1.0 μm. The wall of the gas vesicle is a non-unit membrane 2 to 3 nm in thickness, bearing very regular striations with a periodicity of 4 nm, oriented more or less at right angles to the long axis of the cylinder. This fine structure could be clearly resolved in isolated gas vesicles prepared from a blue-green alga and from Halobacterium halobium, and its presence in the gas vesicles of the green bacterium Pelodictyon clathratiforme was inferred from thin sections. The gas vacuole thus appears to be a homologous organelle in all of these procaryotic groups. Minor differences with respect to the length and arrangement of the gas vesicles were observed. In blue-green algae and green bacteria, the vesicles are relatively long and tend to be arrayed in parallel bundles; in purple sulfur bacteria and Halobacterium, they are shorter and more irregularly distributed in the cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen M. M., Stanier R. Y. Selective isolation of blue-green algae from water and soil. J Gen Microbiol. 1968 Apr;51(2):203–209. doi: 10.1099/00221287-51-2-203. [DOI] [PubMed] [Google Scholar]
  2. Bowen C. C., Jensen T. E. Blue-Green Algae: Fine Structure of the Gas Vacuoles. Science. 1965 Mar 19;147(3664):1460–1462. doi: 10.1126/science.147.3664.1460. [DOI] [PubMed] [Google Scholar]
  3. COHEN-BAZIRE G., PFENNIG N., KUNISAWA R. THE FINE STRUCTURE OF GREEN BACTERIA. J Cell Biol. 1964 Jul;22:207–225. doi: 10.1083/jcb.22.1.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HOUWINK A. L. Flagella, gas vacuoles and cell-wall structure in Halobacterium halobium; an electron microscope study. J Gen Microbiol. 1956 Aug;15(1):146–150. doi: 10.1099/00221287-15-1-146. [DOI] [PubMed] [Google Scholar]
  5. Jost M., Matile P. Zur Charakterisierung der Gasvacuolen der Blaualge Oscillatoria rubescens. Arch Mikrobiol. 1966 Feb 1;53(1):50–58. [PubMed] [Google Scholar]
  6. Larsen H., Omang S., Steensland H. On the gas vacuoles of the halobacteria. Arch Mikrobiol. 1967;59(1):197–203. doi: 10.1007/BF00406332. [DOI] [PubMed] [Google Scholar]
  7. MILLONIG G. A modified procedure for lead staining of thin sections. J Biophys Biochem Cytol. 1961 Dec;11:736–739. doi: 10.1083/jcb.11.3.736. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PRINGSHEIM E. G. OSCILLATORIA AGARDHII VAR. SUSPENSA NOV. VAR. KLEINE MITTEILUNGEN UEBER FLAGELLATEN UND ALGEN. X. Arch Mikrobiol. 1965 Apr 12;50:401–413. doi: 10.1007/BF00509581. [DOI] [PubMed] [Google Scholar]
  9. Pfennig N. Photosynthetic bacteria. Annu Rev Microbiol. 1967;21:285–324. doi: 10.1146/annurev.mi.21.100167.001441. [DOI] [PubMed] [Google Scholar]
  10. Pfennig N., cohen-Bazire G. Some properties of the green bacterium Pelodictyon clathratiforme. Arch Mikrobiol. 1967;59(1):226–236. doi: 10.1007/BF00406336. [DOI] [PubMed] [Google Scholar]
  11. RYTER A., KELLENBERGER E., BIRCHANDERSEN A., MAALOE O. Etude au microscope électronique de plasmas contenant de l'acide désoxyribonucliéique. I. Les nucléoides des bactéries en croissance active. Z Naturforsch B. 1958 Sep;13B(9):597–605. [PubMed] [Google Scholar]
  12. Staley J. T. Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol. 1968 May;95(5):1921–1942. doi: 10.1128/jb.95.5.1921-1942.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Toeckenius W., Kunau W. H. Further characterization of particulate fractions from lysed cell envelopes of Halobacterium halobium and isolation of gas vacuole membranes. J Cell Biol. 1968 Aug;38(2):337–357. doi: 10.1083/jcb.38.2.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Waaland J. R., Branton D. Gas vacuole development in a blue-green alga. Science. 1969 Mar 21;163(3873):1339–1341. doi: 10.1126/science.163.3873.1339. [DOI] [PubMed] [Google Scholar]

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