Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1969 Nov;100(2):1131–1132. doi: 10.1128/jb.100.2.1131-1132.1969

Method for Assessing the Frequency of Transfer of an R Factor on Solid Media and for Isolating Cells of the Donor Type from a Predominantly Repressed Culture

Pat A Lee 1, M H Richmond 1
PMCID: PMC250209  PMID: 4902388

Abstract

A method for assessing the incidence of R factor transfer on solid media is described. The method gives values similar to those obtained with conventional techniques but allows the derepressed cells from a predominantly repressed population to be isolated.

Full text

PDF
1131

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. S., Lewis M. J. Characterization of a transfer factor associated with drug resistance in Salmonella typhimurium. Nature. 1965 Nov 27;208(5013):843–849. doi: 10.1038/208843a0. [DOI] [PubMed] [Google Scholar]
  2. Datta N., Lawn A. M., Meynell E. The relationship of F type piliation and F phage sensitivity to drug resistance transfer in R+F- Escherichia coli K 12. J Gen Microbiol. 1966 Nov;45(2):365–376. doi: 10.1099/00221287-45-2-365. [DOI] [PubMed] [Google Scholar]
  3. GAREN A., LEVINTHAL C. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase. Biochim Biophys Acta. 1960 Mar 11;38:470–483. doi: 10.1016/0006-3002(60)91282-8. [DOI] [PubMed] [Google Scholar]
  4. Lewis M. J. Multiple transmissible drug resistance in an outbreak of Shigella flexneri infection. Lancet. 1967 Nov 4;2(7523):953–956. doi: 10.1016/s0140-6736(67)90793-3. [DOI] [PubMed] [Google Scholar]
  5. Meynell E., Meynell G. G., Datta N. Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids. Bacteriol Rev. 1968 Mar;32(1):55–83. doi: 10.1128/br.32.1.55-83.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Moorhouse E. C. E. coli antibiotic resistance transfer. Ir J Med Sci. 1966 Sep;6(489):375–378. doi: 10.1007/BF02962413. [DOI] [PubMed] [Google Scholar]
  7. Moorhouse E. C., McKay L. Hospital study of transferable drug resistance. Br Med J. 1968 Jun 22;2(5607):741–742. doi: 10.1136/bmj.2.5607.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
  9. TORRIANI A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta. 1960 Mar 11;38:460–469. doi: 10.1016/0006-3002(60)91281-6. [DOI] [PubMed] [Google Scholar]
  10. WATANABE T., FUKASAWA T. Episome-mediated transfer of drug resistance in Enterobacteriaceae. I. Transfer of resistance factors by conjugation. J Bacteriol. 1961 May;81:669–678. doi: 10.1128/jb.81.5.669-678.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES