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. 1969 Nov;100(2):1144–1146. doi: 10.1128/jb.100.2.1144-1146.1969

Aminoglycoside Antibiotics: Inactivation by Phosphorylation in Escherichia coli Carrying R Factors

B Ozanne 1, R Benveniste 1, D Tipper 1, J Davies 1
PMCID: PMC250215  PMID: 4902391

Abstract

R-factor strains which are streptomycin-resistant and spectinomycin-sensitive have been found to inactivate streptomycin by a new mechanism. The 3′-OH group of the 2-deoxy-2-methylamino-l-glucopyranosyl moiety is phosphorylated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Harwood J. H., Smith D. H. Resistance factor-mediated streptomycin resistance. J Bacteriol. 1969 Mar;97(3):1262–1271. doi: 10.1128/jb.97.3.1262-1271.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Kondo S., Okanishi M., Utahara R., Maeda K., Umezawa H. Isolation of kanamycin and paromamine inactivated by E. coli carrying R factor. J Antibiot (Tokyo) 1968 Jan;21(1):22–29. doi: 10.7164/antibiotics.21.22. [DOI] [PubMed] [Google Scholar]
  3. Nossal N. G., Heppel L. A. The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J Biol Chem. 1966 Jul 10;241(13):3055–3062. [PubMed] [Google Scholar]
  4. Okamoto S., Suzuki Y. Chloramphenicol-, dihydrostreptomycin-, and kanamycin-inactivating enzymes from multiple drug-resistant Escherichia coli carrying episome 'R'. Nature. 1965 Dec 25;208(5017):1301–1303. doi: 10.1038/2081301a0. [DOI] [PubMed] [Google Scholar]
  5. Okanishi K., Kondo S., Utahara R., Umezawa H. Phosphorylation and inactivation of aminoglycosidic antibiotics by E. coli carrying R factor. J Antibiot (Tokyo) 1968 Jan;21(1):13–21. doi: 10.7164/antibiotics.21.13. [DOI] [PubMed] [Google Scholar]
  6. Okanishi M., Kondo S., Suzuki Y., Okamoto S., Umezawa H. Studies on inactivation of kanamycin and resistances of E. coli. J Antibiot (Tokyo) 1967 Jul;20(3):132–135. [PubMed] [Google Scholar]
  7. Takasawa S., Utahara R., Okanishi M., Maeda K., Umezawa H. Studies on adenylylstreptomycin, a product of streptomycin inactivation by E. coli carrying the R-factor. J Antibiot (Tokyo) 1968 Aug;21(8):477–484. doi: 10.7164/antibiotics.21.477. [DOI] [PubMed] [Google Scholar]
  8. Umezawa H., Okanishi M., Kondo S., Hamana K., Utahara R., Maeda K., Mitsuhashi S. Phosphorylative inactivation of aminoglycosidic antibiotics by Escherichia coli carrying R factor. Science. 1967 Sep 29;157(3796):1559–1561. [PubMed] [Google Scholar]
  9. Umezawa H., Okanishi M., Utahara R., Maeda K., Kondo S. Isolation and structure of kanamycin inactivated by a cell free system of kanamycin-resistant E. coli. J Antibiot (Tokyo) 1967 Jul;20(3):136–141. [PubMed] [Google Scholar]
  10. Umezawa H., Takasawa S., Okanishi M., Utahara R. Adenylylstreptomycin, a product of streptomycin inactivated by E. coli carrying R factor. J Antibiot (Tokyo) 1968 Jan;21(1):81–82. doi: 10.7164/antibiotics.21.81. [DOI] [PubMed] [Google Scholar]
  11. Yamada T., Tipper D., Davies J. Enzymatic inactivation of streptomycin by R factor-resistant Escherichia coli. Nature. 1968 Jul 20;219(5151):288–291. doi: 10.1038/219288a0. [DOI] [PubMed] [Google Scholar]

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