Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1991 Nov;65(11):5968–5974. doi: 10.1128/jvi.65.11.5968-5974.1991

Relation of VP7 amino acid sequence to monoclonal antibody neutralization of rotavirus and rotavirus monotype.

B S Coulson 1, C Kirkwood 1
PMCID: PMC250261  PMID: 1656083

Abstract

The neutralization epitopes of the VP7 of human rotavirus RV-4 were studied by using five neutralizing mouse monoclonal antibodies to select virus variants resistant to neutralization by each of the antibodies. Antibody resistance patterns and sequence analysis of the RV-4 variants revealed that at least four sites on VP7, located at amino acids 94 (region A), 147 to 148 (region B), 213 (region C), and 291, are involved in neutralization of the human G1 rotavirus RV-4. The A-region site elicited antibody cross-reactive between G types and showed species-restricted immunodominance not related to carbohydrate attachment. The monotype 1b rotavirus M37 lacked this site. The B region contained strain-specific and cross-reactive sites, absent in monotype 1c rotaviruses. The C-region site was present in all G1 rotaviruses tested. Monotype 1a rotaviruses contained all these sites of neutralization. Virus monotype and sensitivity to monoclonal antibody neutralization usually related to the presence of a particular amino acid(s) at or next to the positions at which the mutations were selected in the virus variants.

Full text

PDF
5968

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albert M. J., Unicomb L. E., Bishop R. F. Cultivation and characterization of human rotaviruses with "super short" RNA patterns. J Clin Microbiol. 1987 Jan;25(1):183–185. doi: 10.1128/jcm.25.1.183-185.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bishop R. F., Unicomb L. E., Barnes G. L. Epidemiology of rotavirus serotypes in Melbourne, Australia, from 1973 to 1989. J Clin Microbiol. 1991 May;29(5):862–868. doi: 10.1128/jcm.29.5.862-868.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Both G. W., Bellamy A. R., Street J. E., Siegman L. J. A general strategy for cloning double-stranded RNA: nucleotide sequence of the Simian-11 rotavirus gene 8. Nucleic Acids Res. 1982 Nov 25;10(22):7075–7088. doi: 10.1093/nar/10.22.7075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown D. W., Mathan M. M., Mathew M., Martin R., Beards G. M., Mathan V. I. Rotavirus epidemiology in Vellore, south India: group, subgroup, serotype, and electrophoretype. J Clin Microbiol. 1988 Nov;26(11):2410–2414. doi: 10.1128/jcm.26.11.2410-2414.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Caust J., Dyall-Smith M. L., Lazdins I., Holmes I. H. Glycosylation, an important modifier of rotavirus antigenicity. Arch Virol. 1987;96(3-4):123–134. doi: 10.1007/BF01320955. [DOI] [PubMed] [Google Scholar]
  6. Clark H. F., Hoshino Y., Bell L. M., Groff J., Hess G., Bachman P., Offit P. A. Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol. 1987 Sep;25(9):1757–1762. doi: 10.1128/jcm.25.9.1757-1762.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Coulson B. S., Fowler K. J., Bishop R. F., Cotton R. G. Neutralizing monoclonal antibodies to human rotavirus and indications of antigenic drift among strains from neonates. J Virol. 1985 Apr;54(1):14–20. doi: 10.1128/jvi.54.1.14-20.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Coulson B. S., Grimwood K., Masendycz P. J., Lund J. S., Mermelstein N., Bishop R. F., Barnes G. L. Comparison of rotavirus immunoglobulin A coproconversion with other indices of rotavirus infection in a longitudinal study in childhood. J Clin Microbiol. 1990 Jun;28(6):1367–1374. doi: 10.1128/jcm.28.6.1367-1374.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Coulson B. S., Tursi J. M., McAdam W. J., Bishop R. F. Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3. Virology. 1986 Oct 30;154(2):302–312. doi: 10.1016/0042-6822(86)90456-3. [DOI] [PubMed] [Google Scholar]
  10. Coulson B. S., Unicomb L. E., Pitson G. A., Bishop R. F. Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses. J Clin Microbiol. 1987 Mar;25(3):509–515. doi: 10.1128/jcm.25.3.509-515.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Coulson B. S. Variation in neutralization epitopes of human rotaviruses in relation to genomic RNA polymorphism. Virology. 1987 Aug;159(2):209–216. doi: 10.1016/0042-6822(87)90457-0. [DOI] [PubMed] [Google Scholar]
  12. Dyall-Smith M. L., Holmes I. H. Sequence homology between human and animal rotavirus serotype-specific glycoproteins. Nucleic Acids Res. 1984 May 11;12(9):3973–3982. doi: 10.1093/nar/12.9.3973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Dyall-Smith M. L., Lazdins I., Tregear G. W., Holmes I. H. Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proc Natl Acad Sci U S A. 1986 May;83(10):3465–3468. doi: 10.1073/pnas.83.10.3465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Estes M. K., Cohen J. Rotavirus gene structure and function. Microbiol Rev. 1989 Dec;53(4):410–449. doi: 10.1128/mr.53.4.410-449.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gerna G., Arista S., Passarani N., Sarasini A., Battaglia M. Electropherotype heterogeneity within serotypes of human rotavirus strains circulating in Italy. Brief report. Arch Virol. 1987;95(1-2):129–135. doi: 10.1007/BF01311340. [DOI] [PubMed] [Google Scholar]
  16. Gorziglia M., Larralde G., Kapikian A. Z., Chanock R. M. Antigenic relationships among human rotaviruses as determined by outer capsid protein VP4. Proc Natl Acad Sci U S A. 1990 Sep;87(18):7155–7159. doi: 10.1073/pnas.87.18.7155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Gorziglia M., Larralde G., Ward R. L. Neutralization epitopes on rotavirus SA11 4fM outer capsid proteins. J Virol. 1990 Sep;64(9):4534–4539. doi: 10.1128/jvi.64.9.4534-4539.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Green K. Y., Hoshino Y., Ikegami N. Sequence analysis of the gene encoding the serotype-specific glycoprotein (VP7) of two new human rotavirus serotypes. Virology. 1989 Feb;168(2):429–433. doi: 10.1016/0042-6822(89)90289-4. [DOI] [PubMed] [Google Scholar]
  19. Green K. Y., Midthun K., Gorziglia M., Hoshino Y., Kapikian A. Z., Chanock R. M., Flores J. Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology. 1987 Nov;161(1):153–159. doi: 10.1016/0042-6822(87)90181-4. [DOI] [PubMed] [Google Scholar]
  20. Green K. Y., Sears J. F., Taniguchi K., Midthun K., Hoshino Y., Gorziglia M., Nishikawa K., Urasawa S., Kapikian A. Z., Chanock R. M. Prediction of human rotavirus serotype by nucleotide sequence analysis of the VP7 protein gene. J Virol. 1988 May;62(5):1819–1823. doi: 10.1128/jvi.62.5.1819-1823.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Grimwood K., Lund J. C., Coulson B. S., Hudson I. L., Bishop R. F., Barnes G. L. Comparison of serum and mucosal antibody responses following severe acute rotavirus gastroenteritis in young children. J Clin Microbiol. 1988 Apr;26(4):732–738. doi: 10.1128/jcm.26.4.732-738.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Gunn P. R., Sato F., Powell K. F., Bellamy A. R., Napier J. R., Harding D. R., Hancock W. S., Siegman L. J., Both G. W. Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis. J Virol. 1985 Jun;54(3):791–797. doi: 10.1128/jvi.54.3.791-797.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Huang J., Nagesha H. S., Dyall-Smith M. L., Holmes I. H. Comparative sequence analysis of VP7 genes from five Australian porcine rotaviruses. Arch Virol. 1989;109(3-4):173–183. doi: 10.1007/BF01311079. [DOI] [PubMed] [Google Scholar]
  24. Hum C. P., Dyall-Smith M. L., Holmes I. H. The VP7 gene of a new G serotype of human rotavirus (B37) is similar to G3 proteins in the antigenic c region. Virology. 1989 May;170(1):55–61. doi: 10.1016/0042-6822(89)90351-6. [DOI] [PubMed] [Google Scholar]
  25. Johnson M. A., Misra R. M., Lardelli M., Messina M., Ephraums C., Reeves P. R., Bolcevic Z., Noel J. S., Hum C. P., Van Mai H. Synthesis in Escherichia coli of the major glycoprotein of human rotavirus: analysis of the antigenic regions. Gene. 1989 Dec 7;84(1):73–81. doi: 10.1016/0378-1119(89)90141-8. [DOI] [PubMed] [Google Scholar]
  26. Kobayashi N., Taniguchi K., Urasawa S. Analysis of the newly identified neutralization epitopes on VP7 of human rotavirus serotype 1. J Gen Virol. 1991 Jan;72(Pt 1):117–124. doi: 10.1099/0022-1317-72-1-117. [DOI] [PubMed] [Google Scholar]
  27. Lazdins I., Sonza S., Dyall-Smith M. L., Coulson B. S., Holmes I. H. Demonstration of an immunodominant neutralization site by analysis of antigenic variants of SA11 rotavirus. J Virol. 1985 Oct;56(1):317–319. doi: 10.1128/jvi.56.1.317-319.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Benfield D. A., Greenberg H. B. Characterization of homotypic and heterotypic VP7 neutralization sites of rhesus rotavirus. Virology. 1988 Aug;165(2):511–517. doi: 10.1016/0042-6822(88)90595-8. [DOI] [PubMed] [Google Scholar]
  29. Mason B. B., Dheer S. K., Hsiao C. L., Zandle G., Kostek B., Rosanoff E. I., Hung P. P., Davis A. R. Sequence of the serotype-specific glycoprotein of the human rotavirus Wa strain and comparison with other human rotavirus serotypes. Virus Res. 1985 Jun;2(4):291–299. doi: 10.1016/0168-1702(85)90026-7. [DOI] [PubMed] [Google Scholar]
  30. Matsuno S., Hasegawa A., Mukoyama A., Inouye S. A candidate for a new serotype of human rotavirus. J Virol. 1985 May;54(2):623–624. doi: 10.1128/jvi.54.2.623-624.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Nagesha H. S., Holmes I. H. New porcine rotavirus serotype antigenically related to human rotavirus serotype 3. J Clin Microbiol. 1988 Feb;26(2):171–174. doi: 10.1128/jcm.26.2.171-174.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Nagesha H. S., Huang J., Hum C. P., Holmes I. H. A porcine rotavirus strain with dual VP7 serotype specificity. Virology. 1990 Mar;175(1):319–322. doi: 10.1016/0042-6822(90)90215-d. [DOI] [PubMed] [Google Scholar]
  33. Nishikawa K., Hoshino Y., Taniguchi K., Green K. Y., Greenberg H. B., Kapikian A. Z., Chanock R. M., Gorziglia M. Rotavirus VP7 neutralization epitopes of serotype 3 strains. Virology. 1989 Aug;171(2):503–515. doi: 10.1016/0042-6822(89)90620-x. [DOI] [PubMed] [Google Scholar]
  34. Richardson M. A., Iwamoto A., Ikegami N., Nomoto A., Furuichi Y. Nucleotide sequence of the gene encoding the serotype-specific antigen of human (Wa) rotavirus: comparison with the homologous genes from simian SA11 and UK bovine rotaviruses. J Virol. 1984 Sep;51(3):860–862. doi: 10.1128/jvi.51.3.860-862.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Shaw R. D., Stoner-Ma D. L., Estes M. K., Greenberg H. B. Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3. J Clin Microbiol. 1985 Aug;22(2):286–291. doi: 10.1128/jcm.22.2.286-291.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Taniguchi K., Hoshino Y., Nishikawa K., Green K. Y., Maloy W. L., Morita Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. J Virol. 1988 Jun;62(6):1870–1874. doi: 10.1128/jvi.62.6.1870-1874.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Taniguchi K., Urasawa T., Kobayashi N., Gorziglia M., Urasawa S. Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern: implication for new G serotype specificity. J Virol. 1990 Nov;64(11):5640–5644. doi: 10.1128/jvi.64.11.5640-5644.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Taniguchi K., Urasawa T., Morita Y., Greenberg H. B., Urasawa S. Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7. J Infect Dis. 1987 Jun;155(6):1159–1166. doi: 10.1093/infdis/155.6.1159. [DOI] [PubMed] [Google Scholar]
  39. Tzipori S., Unicomb L., Bishop R., Montenaro J., Vaelioja L. M. Studies on attenuation of rotavirus. A comparison in piglets between virulent virus and its attenuated derivative. Arch Virol. 1989;109(3-4):197–205. doi: 10.1007/BF01311081. [DOI] [PubMed] [Google Scholar]
  40. Unicomb L. E., Coulson B. S., Bishop R. F. Experience with an enzyme immunoassay for serotyping human group A rotaviruses. J Clin Microbiol. 1989 Mar;27(3):586–588. doi: 10.1128/jcm.27.3.586-588.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Wyatt R. G., Greenberg H. B., James W. D., Pittman A. L., Kalica A. R., Flores J., Chanock R. M., Kapikian A. Z. Definition of human rotavirus serotypes by plaque reduction assay. Infect Immun. 1982 Jul;37(1):110–115. doi: 10.1128/iai.37.1.110-115.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES