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. 1991 Nov;65(11):6339–6342. doi: 10.1128/jvi.65.11.6339-6342.1991

Use of recombinant retroviruses to characterize the activity of antiretroviral compounds.

R K Stair 1, C J Nelson 1, J W Mellors 1
PMCID: PMC250351  PMID: 1920637

Abstract

This report describes the use of a recombinant murine retrovirus encoding beta-galactosidase (PLJ beta-gal retrovirus) to study the antiretroviral activity of zidovudine (AZT) and other nucleoside analogs. The PLJ beta-gal virus permits the rapid and unequivocal identification of individual virus-infected cells arising from a single cycle of viral replication. With this model system, AZT is shown to completely and irreversibly prevent retrovirus infection of proliferating cell lines as measured by a lack of reporter gene expression. On the other hand, AZT is less effective in protecting growth-arrested cells from retroviral infection. Recombinant retroviruses such as the PLJ beta-gal virus are potentially useful reagents for the identification and characterization of antiretroviral compounds.

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Selected References

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