Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1991 Nov;65(11):6362–6364. doi: 10.1128/jvi.65.11.6362-6364.1991

Induction of alpha interferon by human immunodeficiency virus type 1 in human monocyte-macrophage cultures.

J Szebeni 1, C Dieffenbach 1, S M Wahl 1, C N Venkateshan 1, A Yeh 1, M Popovic 1, S Gartner 1, L M Wahl 1, M Peterfy 1, R M Friedman 1, et al.
PMCID: PMC250359  PMID: 1920639

Abstract

The induction of interferon (IFN) by human immunodeficiency virus type 1 (HIV-1) in primary, nonstimulated monocyte-macrophage cultures was studied. HIV-1 infection, as confirmed by p24 antigen levels in the cell supernatant, led to the production of alpha interferon (IFN-alpha) over 7 to 21 days following infection. In two of seven experiments, the IFN detected was acid labile. Coupled reverse transcription-polymerase chain reaction analysis confirmed the induction of IFN-alpha mRNA in cells of HIV-1-infected cultures.

Full text

PDF
6362

Images in this article

6363Image
on p.6363

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abb J. Serum interferon and clinical manifestations of infection with human T-lymphotropic virus type III. Med Microbiol Immunol. 1985;174(4):205–210. doi: 10.1007/BF02123697. [DOI] [PubMed] [Google Scholar]
  2. Boumpas D. T., Hooks J. J., Popovic M., Tsokos G. C., Mann D. L. Human T-cell leukemia/lymphoma virus I and/or Epstein-Barr virus-infected B-cell lines spontaneously produce acid-labile alpha-interferon. J Clin Immunol. 1985 Sep;5(5):340–344. doi: 10.1007/BF00918253. [DOI] [PubMed] [Google Scholar]
  3. Capobianchi M. R., De Marco F., Di Marco P., Dianzani F. Acid-labile human interferon alpha production by peripheral blood mononuclear cells stimulated by HIV-infected cells. Arch Virol. 1988;99(1-2):9–19. doi: 10.1007/BF01311019. [DOI] [PubMed] [Google Scholar]
  4. DeStefano E., Friedman R. M., Friedman-Kien A. E., Goedert J. J., Henriksen D., Preble O. T., Sonnabend J. A., Vilcek J. Acid-labile human leukocyte interferon in homosexual men with Kaposi's sarcoma and lymphadenopathy. J Infect Dis. 1982 Oct;146(4):451–459. doi: 10.1093/infdis/146.4.451. [DOI] [PubMed] [Google Scholar]
  5. Eyster M. E., Goedert J. J., Poon M. C., Preble O. T. Acid-labile alpha interferon. A possible preclinical marker for the acquired immunodeficiency syndrome in hemophilia. N Engl J Med. 1983 Sep 8;309(10):583–586. doi: 10.1056/NEJM198309083091003. [DOI] [PubMed] [Google Scholar]
  6. Gartner S., Markovits P., Markovitz D. M., Betts R. F., Popovic M. Virus isolation from and identification of HTLV-III/LAV-producing cells in brain tissue from a patient with AIDS. JAMA. 1986 Nov 7;256(17):2365–2371. [PubMed] [Google Scholar]
  7. Gartner S., Markovits P., Markovitz D. M., Kaplan M. H., Gallo R. C., Popovic M. The role of mononuclear phagocytes in HTLV-III/LAV infection. Science. 1986 Jul 11;233(4760):215–219. doi: 10.1126/science.3014648. [DOI] [PubMed] [Google Scholar]
  8. Gendelman H. E., Friedman R. M., Joe S., Baca L. M., Turpin J. A., Dveksler G., Meltzer M. S., Dieffenbach C. A selective defect of interferon alpha production in human immunodeficiency virus-infected monocytes. J Exp Med. 1990 Nov 1;172(5):1433–1442. doi: 10.1084/jem.172.5.1433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gendelman H. E., Orenstein J. M., Baca L. M., Weiser B., Burger H., Kalter D. C., Meltzer M. S. The macrophage in the persistence and pathogenesis of HIV infection. AIDS. 1989 Aug;3(8):475–495. doi: 10.1097/00002030-198908000-00001. [DOI] [PubMed] [Google Scholar]
  10. Hayes M. P., Enterline J. C., Gerrard T. L., Zoon K. C. Regulation of interferon production by human monocytes: requirements for priming for lipopolysaccharide-induced production. J Leukoc Biol. 1991 Aug;50(2):176–181. doi: 10.1002/jlb.50.2.176. [DOI] [PubMed] [Google Scholar]
  11. Kalter D. C., Nakamura M., Turpin J. A., Baca L. M., Hoover D. L., Dieffenbach C., Ralph P., Gendelman H. E., Meltzer M. S. Enhanced HIV replication in macrophage colony-stimulating factor-treated monocytes. J Immunol. 1991 Jan 1;146(1):298–306. [PubMed] [Google Scholar]
  12. Merrill J. E., Koyanagi Y., Chen I. S. Interleukin-1 and tumor necrosis factor alpha can be induced from mononuclear phagocytes by human immunodeficiency virus type 1 binding to the CD4 receptor. J Virol. 1989 Oct;63(10):4404–4408. doi: 10.1128/jvi.63.10.4404-4408.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Minagawa T., Mizuno K., Hirano S., Asano M., Numata A., Kohanawa M., Nakane A., Hachimori K., Tamagawa S., Negishi M. Detection of high levels of immunoreactive human beta-1 interferon in sera from HIV-infected patients. Life Sci. 1989;45(11):iii–vii. doi: 10.1016/0024-3205(89)90147-1. [DOI] [PubMed] [Google Scholar]
  14. Molina J. M., Scadden D. T., Byrn R., Dinarello C. A., Groopman J. E. Production of tumor necrosis factor alpha and interleukin 1 beta by monocytic cells infected with human immunodeficiency virus. J Clin Invest. 1989 Sep;84(3):733–737. doi: 10.1172/JCI114230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pomerantz R. J., Hirsch M. S. Interferon and human immunodeficiency virus infection. Interferon. 1987;9:113–127. [PubMed] [Google Scholar]
  16. Preble O. T., Rook A. H., Quinnan G. V., Vilcek J., Friedman R. M., Steis R., Gelmann E. P., Sonnabend J. A. Role of interferon in AIDS. Ann N Y Acad Sci. 1984;437:65–75. doi: 10.1111/j.1749-6632.1984.tb37123.x. [DOI] [PubMed] [Google Scholar]
  17. Rossol S., Voth R., Laubenstein H. P., Müller W. E., Schröder H. C., Meyer zum Büschenfelde K. H., Hess G. Interferon production in patients infected with HIV-1. J Infect Dis. 1989 May;159(5):815–821. doi: 10.1093/infdis/159.5.815. [DOI] [PubMed] [Google Scholar]
  18. Roy S., Wainberg M. A. Role of the mononuclear phagocyte system in the development of acquired immunodeficiency syndrome (AIDS). J Leukoc Biol. 1988 Jan;43(1):91–97. doi: 10.1002/jlb.43.1.91. [DOI] [PubMed] [Google Scholar]
  19. Szebeni J., Wahl S. M., Popovic M., Wahl L. M., Gartner S., Fine R. L., Skaleric U., Friedmann R. M., Weinstein J. N. Dipyridamole potentiates the inhibition by 3'-azido-3'-deoxythymidine and other dideoxynucleosides of human immunodeficiency virus replication in monocyte-macrophages. Proc Natl Acad Sci U S A. 1989 May;86(10):3842–3846. doi: 10.1073/pnas.86.10.3842. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wahl L. M., Katona I. M., Wilder R. L., Winter C. C., Haraoui B., Scher I., Wahl S. M. Isolation of human mononuclear cell subsets by counterflow centrifugal elutriation (CCE). I. Characterization of B-lymphocyte-, T-lymphocyte-, and monocyte-enriched fractions by flow cytometric analysis. Cell Immunol. 1984 May;85(2):373–383. doi: 10.1016/0008-8749(84)90251-x. [DOI] [PubMed] [Google Scholar]
  21. Wahl S. M., Allen J. B., McCartney-Francis N., Morganti-Kossmann M. C., Kossmann T., Ellingsworth L., Mai U. E., Mergenhagen S. E., Orenstein J. M. Macrophage- and astrocyte-derived transforming growth factor beta as a mediator of central nervous system dysfunction in acquired immune deficiency syndrome. J Exp Med. 1991 Apr 1;173(4):981–991. doi: 10.1084/jem.173.4.981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wahl S. M., Katona I. M., Stadler B. M., Wilder R. L., Helsel W. E., Wahl L. M. Isolation of human mononuclear cell subsets by counterflow centrifugal elutriation (CCE). II. Functional properties of B-lymphocyte-, T-lymphocyte-, and monocyte-enriched fractions. Cell Immunol. 1984 May;85(2):384–395. doi: 10.1016/0008-8749(84)90252-1. [DOI] [PubMed] [Google Scholar]
  23. Yeh T. J., McBride P. T., Overall J. C., Jr, Green J. A. Automated, quantitative cytopathic effect reduction assay for interferon. J Clin Microbiol. 1982 Aug;16(2):413–415. doi: 10.1128/jcm.16.2.413-415.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES