Abstract
A 3-ketosucrose-degrading enzyme was purified 80-fold from the sonic extracts of Agrobacterium tumefaciens IAM 1525 grown on a sucrose-containing medium. The enzyme catalyzes hydrolysis of α-3-ketoglucosides such as 3-ketosucrose, 3-ketotrehalose, 3-ketomaltose, and 3-ketoglucose-1-phosphate but not of β-3-ketoglucosides, β-3-ketogalactosides, and other glycosides such as sucrose, trehalose, maltose, glucose-1-phosphate, cellobiose, lactose, or raffinose. From the strict substrate specificity of this enzyme, the name α-d-3-ketoglucoside 3-ketoglucohydrolase (trivial name, α-3-ketoglucosidase) was proposed. Km values for 3-ketosucrose and 3-ketotrehalose were 3.9 × 10−3m and 4.8 × 10−3m, respectively. Optimum pH was 8.0 to 8.3. 3-Ketoglucose, a reaction product from α-3-ketoglucosides by the enzyme, behaved as a strong inhibitor. Physiological significance of this enzyme in the disaccharide metabolism of this bacterium was discussed.
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