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. 1970 Mar;101(3):844–850. doi: 10.1128/jb.101.3.844-850.1970

Nature of the Ethylenediaminetetraacetic acid Requirement for Transformation of Bacillus subtilis with Single-Stranded Deoxyribonucleic Acid

M J Tevethia 1, M Mandel 1
PMCID: PMC250400  PMID: 4191319

Abstract

The ethylenediaminetetraacetate (EDTA) requirement for transformation of Bacillus subtilis with single-stranded deoxyribonucleic acid (DNA) was examined. The results indicate that a chelating agent such as EDTA is a stringent requirement for transformation with single DNA strands only at nonsaturating DNA concentrations, and that EDTA, when required, must be present during several steps in the transformation process and appears to insure the survival of single-stranded DNA by rendering a nuclease in competent populations inactive.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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