Analysis of mineralized matrix deposited on H-terminated USND, Ti-6Al-4V and CoCrMo. Osteoblastic differentiation of MSCs adherent to the metal substrates was induced by culture for 4 weeks in an osteogenic media (OS+). The cells were allowed to deposit mineral on the biomaterial surfaces, and then the calcium content was quantified at week 4. Cells were also cultured on tissue culture plastic in osteogenic media (OS+, positive control) or in normal growth media (OS-, negative control). Slightly more mineral was deposited on Ti-6Al-4V than USND, but the difference in mineral deposition between USND and CoCrMo surfaces was not significant. * denotes p< 0.05