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. 1970 Mar;101(3):921–924. doi: 10.1128/jb.101.3.921-924.1970

Isolation of Mutants of Staphylococcus aureus Lacking Extracellular Nuclease Activity1

Gilbert S Omenn a,2, John Friedman a,3
PMCID: PMC250411  PMID: 4985592

Abstract

Suspensions of Staphylococcus aureus Foggi strain were exposed to nitrosoguanidine and screened on deoxyribonucleic acid-acridine orange-agar plates for loss of extracellular nuclease activity. All nuclease-deficient mutants lacked cross-reacting material when tested with antinuclease antibody. Coagulase and β-hemolysin were also lost in nuclease-deficient mutants, and all three enzyme functions were regained together upon reversion with ethyl methane sulfonate. These observations are consistent with the suggestion that the synthesis or the release, or both the synthesis and release, of certain extracellular enzymes may be subject to coordinated control mechanisms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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