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. 1970 Mar;101(3):931–940. doi: 10.1128/jb.101.3.931-940.1970

Biochemical Changes During Fungal Sporulation and Spore Germination

I. Phenyl Methyl Sulfonyl Fluoride Inhibition of Macroconidial Germination in Microsporum gypseum1

T J Leighton a, J J Stock a
PMCID: PMC250413  PMID: 4191321

Abstract

Macroconidia of Microsporum gypseum release free amino acids into the medium during germination. A single alkaline protease is also found in the germination supernatant fraction. The purified protease is capable of hydrolyzing isolated spore coats in vitro. Phenyl methyl sulfonyl fluoride (PMSF) is an effective inhibitor of the protease. Incorporation of PMSF at 10−4m into the germination system inhibits spore germination and the release of free amino nitrogen. Addition of PMSF after germ tube emergence is completed has no effect on subsequent outgrowth. The addition of exogenous purified protease to quiescent spores results in more than a 2.5-fold increase in germinated spores. It is concluded that spore coat proteolysis is an essential event in the germination of dermatophyte macroconidia. A model system to explain macroconidial germination response to inhibition, temperature shift, and addition of protease is presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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