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. 1970 Mar;101(3):959–964. doi: 10.1128/jb.101.3.959-964.1970

Control of Uridine Diphosphate-Glucose Dehydrogenase Synthesis and Uridine Diphosphate-Glucuronic Acid Accumulation by a Regulator Gene Mutation in Escherichia coli K-12

Michael M Lieberman a,1, Anna Shaparis a, Alvin Markovitz a
PMCID: PMC250416  PMID: 4908789

Abstract

Uridine diphosphate (UDP)-glucose dehydrogenase, the enzyme that converts UDP-glucose to UDP-glucuronic acid, was derepressed in a mucoid (capR9) strain of Escherichia coli K-12 and repressed in a nonmucoid (capR+) strain. A nonmucoid mutant (strain MC 152; capR9 non-2) derived from the mucoid strain accumulated large quantities of nucleotides. Among these nucleotides, UDP-glucuronic acid was identified as well as guanosine triphosphate and an adenosine diphosphate-sugar. UDP-glucose dehydrogenase was still derepressed in strain MC 152. When the nonmucoid mutant was transduced to the wild-type state for this regulator gene (capR+), the transductant was found to accumulate less total nucleotides, and the accumulation of UDP-glucuronic acid was abolished. UDP-glucose dehydrogenase was repressed in the capR+non-2 strain but not to the same extent that it was in the capR+ strain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AXELROD J., KALCKAR H. M., MAXWELL E. S., STROMINGER J. L. Enzymatic formation of uridine diphosphoglucuronic acid. J Biol Chem. 1957 Jan;224(1):79–90. [PubMed] [Google Scholar]
  2. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  3. GINSBURG V. Studies on the biosynthesis of guanosine diphosphate L-fucose. J Biol Chem. 1961 Sep;236:2389–2393. [PubMed] [Google Scholar]
  4. Kang S., Markovitz A. Induction of capsular polysaccharide synthesis by rho-fluorophenylalanine in Escherichia coli wild type and strains with altered phenylalanyl soluble ribonucleic acid synthetase. J Bacteriol. 1967 Feb;93(2):584–591. doi: 10.1128/jb.93.2.584-591.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Knutson C. A., Jeanes A. A new modification of the carbazole analysis: application to heteropolysaccharides. Anal Biochem. 1968 Sep;24(3):470–481. doi: 10.1016/0003-2697(68)90154-1. [DOI] [PubMed] [Google Scholar]
  6. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  7. Lieberman M. M., Markovitz A. Depression of guanosine diphosphate-mannose pyrophosphorylase by mutations in two different regulator genes involved in capsular polysaccharide synthesis in Escherichia coli K-12. J Bacteriol. 1970 Mar;101(3):965–972. doi: 10.1128/jb.101.3.965-972.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MARKOVITZ A., CIFONELLI J. A., DORFMAN A. The biosynthesis of hyaluronic acid by group A Streptococcus. VI. Biosynthesis from uridine nucleotides in cell-free extracts. J Biol Chem. 1959 Sep;234:2343–2350. [PubMed] [Google Scholar]
  9. MARKOVITZ A. REGULATORY MECHANISMS FOR SYNTHESIS OF CAPSULAR POLYSACCHARIDE IN MUCOID MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1964 Feb;51:239–246. doi: 10.1073/pnas.51.2.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Markovitz A., Baker B. Suppression of radiation sensitivity and capsular polysaccharide synthesis in Escherichia coli K-12 by ochre suppressors. J Bacteriol. 1967 Aug;94(2):388–395. doi: 10.1128/jb.94.2.388-395.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Markovitz A., Lieberman M. M., Rosenbaum N. Derepression of phosphomannose isomerase by regulator gene mutations involved in capsular polysaccharide synthesis in Escherichia coli K-12. J Bacteriol. 1967 Nov;94(5):1497–1501. doi: 10.1128/jb.94.5.1497-1501.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Markovitz A., Rosenbaum N. A regulator gene that is dominant on an episome and recessive on a chromosome. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1084–1091. doi: 10.1073/pnas.54.4.1084. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. PARK J. T., JOHNSON M. J. A submicrodetermination of glucose. J Biol Chem. 1949 Nov;181(1):149–151. [PubMed] [Google Scholar]
  14. SMITH E. E., MILLS G. T., BERNHEIMER H. P., AUSTRIAN R. The formation of uridine pyrophosphoglucuronic acid from uridine pyrophosphoglucose by extracts of a noncapsulated strain of pneumococcus. Biochim Biophys Acta. 1958 Apr;28(1):211–212. doi: 10.1016/0006-3002(58)90455-4. [DOI] [PubMed] [Google Scholar]

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