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. 1970 Mar;101(3):1073–1075. doi: 10.1128/jb.101.3.1073-1075.1970

Thiostrepton, an Inhibitor of 50S Ribosome Subunit Function

B Weisblum 1, V Demohn 1
PMCID: PMC250429  PMID: 4985587

Abstract

Thiostrepton inhibits 14C-leucine incorporation by intact cells of Bacillus megaterium as well as 14C-phenylalanine incorporation by a poly U-directed extract of Escherichia coli. Extracts of E. coli which are pretreated by incubation with thiostrepton cannot be reactivated by dialysis to more than 5% of their former activity. The 50S ribosome subunit appears to be the site of thiostrepton action, since protein-synthesizing activity can be restored to dialyzed pretreated extracts by supplementation with 50S ribosome subunits but not with 30S ribosome subunits. This technique also provides a simple sensitive method for detection of the biological activity of very small amounts of 50S ribosome subunits.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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