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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Virology. 2008 Jun 5;377(2):379–390. doi: 10.1016/j.virol.2008.04.034

Figure 2. Western blot (WB) detection of pp65 and IEfusion protein antigens.

Figure 2

A) Lane 1: CEF cell lysate infected with pp65-rMVA as (+) control; Lanes 2 and 3: cell lysate from wtMVA infected and uninfected CEF as (-) controls; Lane 4: cell lysate of pp65-IEfusion-MVA infected CEF cells. The WB in Panel A was incubated with mAB 28-103 against pp65. B) Lane 5: cell lysate of CEF infected with rMVA expressing IE1/e4 as (+) control; Lanes 6 and 7: cell lysate from wtMVA infected and uninfected CEF as (-) controls; Lane 8: cell lysate of pp65-IEfusion-MVA infected CEF cells and Lane 9: cell lysate of IEfusion-MVA infected CEF cells. The WB in Panel B was incubated with mAB p63-27 against IE1. See M&M for details of MVA construction that clarifies that pp65 and IEfusion are separate proteins of 65 and 125 Kda, respectively.