Abstract
The effects of temperature on the activation, glucose-induced germination, and outgrowth of Bacillus megaterium QM B1551 spores were investigated. There was no evidence for discontinuities in the response of spores to temperature in these processes reflecting reported thermal anomalies in the physical structure of water. Increasing the temperature of heat activation (aqueous suspensions, 5 min) increased the germinability of spores. Activation, as measured by extent of germination, was optimal after heating at 62 to 78 C, and the rate of spore germination was maximal after heat activation at 64 to 68 C. Increasing the temperature of activation above 68 C depressed the germination rate and increased the time lag before this rate was reached. Germination occurred over a wide range of temperatures, but was optimal between 28 and 38 C. The highest rate of germination was at 38 C; at lower incubation temperatures, the maximum attained rate was lower and the lag in attaining this rate was extended. Outgrowth (postgerminative development through the first cell division) of the germinated spores in Brain Heart Infusion (BHI) occurred in at least two phases—a temperature-dependent lag phase followed by a relatively temperature-independent phase of maximum outgrowth rate, during which increase in optical density was a linear function of time. Outgrowth time (time required for doubling of the initial optical density), essentially dependent on the time for completion of the lag phase, was shortest at temperatures between 34 and 40 C. The temperature-dependent lag phase was completed in a rich medium (e.g., BHI) but not in the glucose germination medium, suggesting that the endogenous reserves of the germinated spore were inadequate to support the metabolic synthetic events occurring during this period.
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- Curran H. R., Evans F. R. Heat Activation Inducing Germination in the Spores of Thermotolerant and Thermophilic Aerobic Bacteria. J Bacteriol. 1945 Apr;49(4):335–346. doi: 10.1128/jb.49.4.335-346.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davey C. B., Miller R. J., Nelson L. A. Temperature-dependent anomalies in the growth of microorganims. J Bacteriol. 1966 May;91(5):1827–1830. doi: 10.1128/jb.91.5.1827-1830.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Falk M., Kell G. S. Thermal properties of water: discontinuities questioned. Science. 1966 Nov 25;154(3752):1013–1015. doi: 10.1126/science.154.3752.1013. [DOI] [PubMed] [Google Scholar]
- Hyatt M. T., Levinson H. S. Water vapor, aqueous ethyl alcohol, and heat activation of Bacillus megaterium spore germination. J Bacteriol. 1968 Jun;95(6):2090–2101. doi: 10.1128/jb.95.6.2090-2101.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KEYNAN A., EVANCHIK Z., HALVORSON H. O., HASTINGS J. W. ACTIVATION OF BACTERIAL ENDOSPORES. J Bacteriol. 1964 Aug;88:313–318. doi: 10.1128/jb.88.2.313-318.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KEYNAN A., HALVORSON H. O. Calcium dipicolinic acid-induced germination of Bacillus cereus spores. J Bacteriol. 1962 Jan;83:100–105. doi: 10.1128/jb.83.1.100-105.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levinson H. S., Hyatt M. T., Holmes P. K. Transition of bacterial spores into vegetative cells. Trans N Y Acad Sci. 1967 Nov;30(1):81–98. doi: 10.1111/j.2164-0947.1967.tb02454.x. [DOI] [PubMed] [Google Scholar]
- O'CONNOR R. J., HALVORSON H. O. L-Alanine dehydrogenase: a mechanism controlling the specificity of amino acid-induced germination of Bacillus cereus spores. J Bacteriol. 1961 Nov;82:706–713. doi: 10.1128/jb.82.5.706-713.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POWELL J. F., HUNTER J. R. Spore germination in the genus bacillus: the modification of germination requirements as a result of preheating. J Gen Microbiol. 1955 Aug;13(1):59–67. doi: 10.1099/00221287-13-1-59. [DOI] [PubMed] [Google Scholar]
- VARY J. C., HALVORSON H. O. KINETICS OF GERMINATION OF BACILLUS SPORES. J Bacteriol. 1965 May;89:1340–1347. doi: 10.1128/jb.89.5.1340-1347.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VAS K., PROSZT G. Effect of temperature and hydrogen-ion concentration on the germination of spores of Bacillus cereus. Nature. 1957 Jun 22;179(4573):1301–1302. doi: 10.1038/1791301a0. [DOI] [PubMed] [Google Scholar]