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. 1970 Jan;101(1):160–165. doi: 10.1128/jb.101.1.160-165.1970

Double-Bond Requirement in a Fatty Acid Desaturase Mutant of Saccharomyces cerevisiae

Bernadine J Wisnieski a,1, Alec D Keith a, Michael R Resnick a,2
PMCID: PMC250465  PMID: 5411750

Abstract

A fatty acid Δ9-desaturase mutant of yeast was analyzed to establish the specificity of the fatty acid structural requirement. Several double-bond-containing and substituted fatty acids were tested. It was concluded that the presence of a Δ9cis double bond is necessary for growth. The need for a specific chain length or a specific number of double bonds, or for both, is more flexible. Tracer-containing 16:1Δ9cis, 18:1Δ9cis, 18:2Δ9,12cis,cis and 18:3Δ9,12,15 all cis revealed that each of these growth-supporting components, once taken up, was not converted into other fatty acids. Concentration effects on doubling time were also considered.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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