Abstract
Addition of the tryptophan analogues 5-methyl-tryptophan, 5-hydroxy-tryptophan, and 7-aza-tryptophan, during tryptophan starvation of a tryptophan auxotroph of Bacillus subtilis, stimulated amino acid incorporation. Two of the analogues tested, 5-methyl-tryptophan and 5-hydroxy-tryptophan, competed with each other, but their addition did not prevent tryptophan incorporation into acid-insoluble material. The incorporation of tryptophan and 5-hydroxy-tryptophan was stimulated when a mixture of phenylalanine and tyrosine was present in the medium. The two analogues 5-methyl-tryptophan and 5-hydroxy-tryptophan were recovered in protein hydrolysates of analogue-grown cultures, and there is evidence suggesting that the analogues are not chain terminators. Neither analogue prevented the polysome degradation that occurs during tryptophan starvation.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- GRIPPO P., IACCARINO M., ROSSI M., SCARANO E. THIN-LAYER CHROMATOGRAPHY OF NUCLEOTIDES, NUCLEOSIDES AND NUCLEIC ACID BASES. Biochim Biophys Acta. 1965 Jan 11;95:1–7. doi: 10.1016/0005-2787(65)90204-2. [DOI] [PubMed] [Google Scholar]
- Jensen R. A. Antimetabolite action of 5-methyltryptophan in Bacillus subtilis. J Bacteriol. 1969 Mar;97(3):1500–1501. doi: 10.1128/jb.97.3.1500-1501.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVIN D. H., THANG M. N., GRUNBERG-MANAGO M. SYNTHESIS IN VIVO OF POLYNUCLEOTIDE PHOSPHORYLASE IN ESCHERICHIA COLI. I. EFFECT OF AMINO ACIDS ON POLYNUCLEOTIDE PHOSPHORYLASE ACTIVITY IN A CHLORAMPHENICOL-INHIBITED SYSTEM. Biochim Biophys Acta. 1963 Dec 20;76:558–571. doi: 10.1016/0006-3002(63)90082-9. [DOI] [PubMed] [Google Scholar]
- Lark K. G. Incorporation of 5-methyltryptophan into the protein of Escherichia coli 15T- (555-7). J Bacteriol. 1969 Feb;97(2):980–982. doi: 10.1128/jb.97.2.980-982.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MOYED H. S. False feedback inhibition: inhibition of tryptophan biosynthesis by 5-methyltryptophan. J Biol Chem. 1960 Apr;235:1098–1102. [PubMed] [Google Scholar]
- MUNIER R., COHEN G. N. Incorporation d'analogues structuraux d'aminoacides dans les protéines bactériennes au cours de leur synthèse in vivo. Biochim Biophys Acta. 1959 Feb;31(2):378–391. doi: 10.1016/0006-3002(59)90011-3. [DOI] [PubMed] [Google Scholar]
- NISMAN B., HIRSCH M. L. Etude de l'activation et de l'incorporation des acides aminées par des fractions enzymatiques d'E. coli. Ann Inst Pasteur (Paris) 1958 Nov;95(5):615–636. [PubMed] [Google Scholar]
- Nester E. W., Jensen R. A. Control of aromatic acid biosynthesis in Bacillus subtilis: sequenial feedback inhibition. J Bacteriol. 1966 Apr;91(4):1594–1598. doi: 10.1128/jb.91.4.1594-1598.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
- THANG M. N., WILLIAMS F. R., GRUNBERG-MANAGO M. SYNTH'ESE IN VIVO DE LA POLYNUCL'EOTIDE PHOSPHORYLASE CHEZ ESCHERICHIA COLI. II. SYNTH'ESE DE NOVO DE LA POLYNUCL'EOTIDE PHOSPHORYLASE EN PRESENCE DE CHLORAMPH'ENICOL. Biochim Biophys Acta. 1963 Dec 20;76:572–588. doi: 10.1016/0006-3002(63)90083-0. [DOI] [PubMed] [Google Scholar]