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. 1970 Jan;101(1):181–187. doi: 10.1128/jb.101.1.181-187.1970

Repression of Phenolic Acid-Synthesizing Enzymes and Its Relation to Iron Uptake in Bacillus subtilis

D N Downer 1, W B Davis 1, B R Byers 1
PMCID: PMC250468  PMID: 4983647

Abstract

Excretion of the metal-chelating phenolic acid, 2,3-dihydroxybenzoate, by a tryptophan-requiring strain (M-13) of Bacillus subtilis was inversely proportional to the iron added to the medium. Addition of iron as the ferric chelates of two secondary hydroxamates (ferri-schizokinen and Desferal) markedly reduced excretion. Synthesis of 2,3-dihydroxybenzoate from chorismate by extracts of B. subtilis M-13, grown in low-iron medium, was unaltered by additions of FeSO4, FeCl3, ferrischizokinen, 2,3-dihydroxybenzoate, the 2,3-dihydroxybenzoate-iron complex, or by extracts of cells grown in high-iron medium (which contained no demonstrable 2,3-dihydroxybenzoate-synthesizing activity) to the extracts of “low-iron cells.” Iron control seemed to involve repression of synthesis of the enzymes in the 2,3-dihydroxybenzoate pathway. Both ferri-schizokinen and 2,3-dihydroxybenzoate plus iron enhanced considerably the otherwise minimal repressive effects of iron at low concentrations. Ferri-schizokinen delayed derepression of the pathway in B. subtilis M-13, and reduced its rate of synthesis after derepression. Addition of FeSO4 to derepressed cells of B. subtilis M-13 halted synthesis of the enzymes after a lag period. The effect of the ferric hydroxamates was related to the capacity of B. subtilis M-13 to incorporate 59Fe3+ from Desferal-59Fe3+. Cellular accumulation of 59Fe3+ from Desferal-59Fe3+ after 20 min was nearly double that incorporated from 59FeCl3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brot N., Goodwin J. Regulation of 2,3-dihydroxybenzoylserine synthetase by iron. J Biol Chem. 1968 Feb 10;243(3):510–513. [PubMed] [Google Scholar]
  2. Byers B. R., Lankford C. E. Regulation of synthesis of 2,3-dihydroxybenzoic acid in Bacillus subtilis by iron and a biological secondary hydroxamate. Biochim Biophys Acta. 1968 Oct 15;165(3):563–566. doi: 10.1016/0304-4165(68)90244-4. [DOI] [PubMed] [Google Scholar]
  3. Byers B. R., Powell M. V., Lankford C. E. Iron-chelating hydroxamic acid (schizokinen) active in initiation of cell division in Bacillus megaterium. J Bacteriol. 1967 Jan;93(1):286–294. doi: 10.1128/jb.93.1.286-294.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gibson F. Chorismic acid: purification and some chemical and physical studies. Biochem J. 1964 Feb;90(2):256–261. doi: 10.1042/bj0900256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gibson F., Pittard J. Pathways of biosynthesis of aromatic amino acids and vitamins and their control in microorganisms. Bacteriol Rev. 1968 Dec;32(4 Pt 2):465–492. [PMC free article] [PubMed] [Google Scholar]
  6. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  7. Knüsel F., Nüesch J., Treichler H. J. Siderochrome und Eisenstoffwechsel bei Mikroorganismen. Naturwissenschaften. 1967 May;54(10):242–247. doi: 10.1007/BF00602138. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lorence J. H., Nester E. W. Multiple molecular forms of chorismate mutase in Bacillus subtillis. Biochemistry. 1967 May;6(5):1541–1553. doi: 10.1021/bi00857a041. [DOI] [PubMed] [Google Scholar]
  10. Neilands J. B. Hydroxamic acids in nature. Science. 1967 Jun 16;156(3781):1443–1447. doi: 10.1126/science.156.3781.1443. [DOI] [PubMed] [Google Scholar]
  11. Nester E. W., Jensen R. A. Control of aromatic acid biosynthesis in Bacillus subtilis: sequenial feedback inhibition. J Bacteriol. 1966 Apr;91(4):1594–1598. doi: 10.1128/jb.91.4.1594-1598.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Peters W. J., Warren R. A. Itoic acid synthesis in Bacillus subtilis. J Bacteriol. 1968 Feb;95(2):360–366. doi: 10.1128/jb.95.2.360-366.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Peters W. J., Warren R. A. Phenolic acids and iron transport in Bacillus subtilis. Biochim Biophys Acta. 1968 Sep 3;165(2):225–232. doi: 10.1016/0304-4165(68)90050-0. [DOI] [PubMed] [Google Scholar]
  14. Young I. G., Cox G. B., Gibson F. 2,3-Dihydroxybenzoate as a bacterial growth factor and its route of biosynthesis. Biochim Biophys Acta. 1967 Jul 25;141(2):319–331. doi: 10.1016/0304-4165(67)90106-7. [DOI] [PubMed] [Google Scholar]
  15. Young I. G., Gibson F. Regulation of the enzymes involved in the biosynthesis of 2,3-dihydroxybenzoic acid in Aerobacter aerogenes and Escherichia coli. Biochim Biophys Acta. 1969 May 6;177(3):401–411. doi: 10.1016/0304-4165(69)90302-x. [DOI] [PubMed] [Google Scholar]

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