Fig. 3.
The effects of ACPT-1 are not occluded by GABAB receptor activation. (A) Single voltage clamp recordings of eEPSCs evoked in response to a 20 Hz train of 20 stimuli in the presence of the GABABR agonist baclofen show a pronounced depression across the train. Subsequently, ACPT-1 in the continued presence of baclofen, elicited a further depression. (B) Pooled mean eEPSC amplitude (n=7) for each event in a 20 Hz train (C) Pooled analysis of mean eEPSC amplitude from all events in the train from seven neurones. The substantial reduction by baclofen does not occlude a further significant depression by ACPT-1. (D) Cumulative probability analysis of sEPSCs in the seven neurones. A dramatic reduction in frequency by baclofen is partially reversed by ACPT-1. Scale bars=150 pA, 225 ms (A).