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. 1970 Jan;101(1):286–291. doi: 10.1128/jb.101.1.286-291.1970

Correlation of Ultrastructure in Azotobacter vinelandii with Nitrogen Source for Growth

Joel Oppenheim 1, Leon Marcus 1
PMCID: PMC250479  PMID: 5411753

Abstract

Azotobacter synthesizes an extensive internal membranous nework when grown with air (N2), i.e., under conditions when these bacteria fix nitrogen. Very slight quantities of internal membrane, concentrated mainly about the cell periphery, are formed when Azotobacter grows with fixed nitrogen, i.e., ammonia and amino acids. Compared to cells growing with ammonia, cells utilizing atmospheric nitrogen as the sole nitrogen source are smaller in size and volume, grow one-third slower, and lack detectable poly-β-hydroxybutyrate.

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Selected References

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  1. ABRAMS A., NIELSEN L., THAEMERT J. RAPIDLY SYNTHESIZED RIBONUCLEIC ACID IN MEMBRANE GHOSTS FROM STREPTOCOCCUS FECALIS PROTOPLASTS. Biochim Biophys Acta. 1964 Feb 17;80:325–337. doi: 10.1016/0926-6550(64)90104-5. [DOI] [PubMed] [Google Scholar]
  2. Aronson A. Adsorption of polysomes to bacterial membranes. J Mol Biol. 1966 Feb;15(2):505–514. doi: 10.1016/s0022-2836(66)80124-9. [DOI] [PubMed] [Google Scholar]
  3. BOATMAN E. S. OBSERVATIONS ON THE FINE STRUCTURE OF SPHEROPLASTS OF RHODOSPIRILLUM RUBRUM. J Cell Biol. 1964 Feb;20:297–311. doi: 10.1083/jcb.20.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bulen W. A., Burns R. C., LeComte J. R. Nitrogen fixation: cell-free system with extracts of Azotobacter. Biochem Biophys Res Commun. 1964 Oct 14;17(3):265–271. doi: 10.1016/0006-291x(64)90395-x. [DOI] [PubMed] [Google Scholar]
  5. COTA-ROBLES E. H., MARR A. G., NILSON E. H. Submicroscopic particles in extracts of Azotobacter agilis. J Bacteriol. 1958 Mar;75(3):243–252. doi: 10.1128/jb.75.3.243-252.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GLAUERT A. M. The fine structure of bacteria. Br Med Bull. 1962 Sep;18:245–250. doi: 10.1093/oxfordjournals.bmb.a069988. [DOI] [PubMed] [Google Scholar]
  7. HENDLER R. W., BANFIELD W. G., TANI J., KUFFEL ON THE CYTOLOGICAL UNIT FOR PROTEIN SYNTHESIS IN VIVO IN E. COLI. III. ELECTRON MICROSCOPIC AND ULTRACENTRIFUGAL EXAMINATION OF INTACT CELLS AND FRACTIONS. Biochim Biophys Acta. 1964 Feb 17;80:307–314. [PubMed] [Google Scholar]
  8. HOLT S. C., MARR A. G. EFFECT OF LIGHT INTENSITY ON THE FORMATION OF INTRACYTOPLASMIC MEMBRANE IN RHODOSPIRILLUM RUBRUM. J Bacteriol. 1965 May;89:1421–1429. doi: 10.1128/jb.89.5.1421-1429.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HOLT S. C., MARR A. G. LOCATION OF CHLOROPHYLL IN RHODOSPIRILLUM RUBRUM. J Bacteriol. 1965 May;89:1402–1412. doi: 10.1128/jb.89.5.1402-1412.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. IMAEDA T., OGURA M. Formation of intracytoplasmic membrane system of mycobacteria related to cell division. J Bacteriol. 1963 Jan;85:150–163. doi: 10.1128/jb.85.1.150-163.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MARQUIS R. E., GERHARDT P. RESPIRATION-COUPLED AND PASSIVE UPTAKE OF ALPHA-AMINOISOBUTYRIC ACID, A METABOLICALLY INERT TRANSPORT ANALOGUE, BY BACILLUS MEGATERIUM. J Biol Chem. 1964 Oct;239:3361–3371. [PubMed] [Google Scholar]
  14. MITCHELL P. Structure and function in microorganisms. Biochem Soc Symp. 1959;16:73–93. [PubMed] [Google Scholar]
  15. Oppenheim J., Fisher R. J., Wilson P. W., Marcus L. Properties of a soluble nitrogenase in Azotobacter. J Bacteriol. 1970 Jan;101(1):292–296. doi: 10.1128/jb.101.1.292-296.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Oppenheim J., Scheinbuks J., Biava C., Marcus L. Polyribosomes in Azotobacter vinelandii. I. Isolation, characterization and distribution of ribosomes, polyribosomes and subunits in logarithmically growing azotobacter. Biochim Biophys Acta. 1968 Jul 23;161(2):386–401. [PubMed] [Google Scholar]
  17. Pangborn J., Marr A. G., Robrish S. A. LOCALIZATION OF RESPIRATORY ENZYMES IN INTRACYTOPLASMIC MEMBRANES OF AZOTOBACTER AGILIS. J Bacteriol. 1962 Oct;84(4):669–678. doi: 10.1128/jb.84.4.669-678.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. ROBRISH S. A., MARR A. G. Location of enzymes in Azotobacteragilis. J Bacteriol. 1962 Jan;83:158–168. doi: 10.1128/jb.83.1.158-168.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. RYTER A., JACOB F. ETUDE AU MICROSCOPE 'ELECTRONIQUE DES RELATIONS ENTRE M'ESOSOMES ET NOYAUX CHEZ BACILLUS SUBTILIS. C R Hebd Seances Acad Sci. 1963 Nov 13;257:3060–3063. [PubMed] [Google Scholar]
  21. RYTER A., LANDMAN O. E. ELECTRON MICROSCOPE STUDY OF THE RELATIONSHIP BETWEEN MESOSOME LOSS AND THE STABLE L STATE (OR PROTOPLAST STATE) IN BACILLUS SUBTILIS. J Bacteriol. 1964 Aug;88:457–467. doi: 10.1128/jb.88.2.457-467.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SCHLESSINGER D. PROTEIN SYNTHESIS BY POLYRIBOSOMES ON PROTOPLAST MEMBRANES OF B. MEGATERIUM. J Mol Biol. 1963 Nov;7:569–582. doi: 10.1016/s0022-2836(63)80103-5. [DOI] [PubMed] [Google Scholar]
  23. TANI J., HENDLER R. W. ON THE CYTOLOGICAL UNIT FOR PROTEIN SYNTHESIS IN VIVO IN E. COLI. I. STUDIES WITH SPHEROPLASTS OF TYPE K-12. Biochim Biophys Acta. 1964 Feb 17;80:279–293. doi: 10.1016/0926-6550(64)90100-8. [DOI] [PubMed] [Google Scholar]
  24. VATTER A. E., WOLFE R. S. The structure of photosynthetic bacteria. J Bacteriol. 1958 Apr;75(4):480–488. doi: 10.1128/jb.75.4.480-488.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. WYSS O., NEUMNN M. G., SOCOLOFSKY M. D. Development and germination of the Azotobacter cyst. J Biophys Biochem Cytol. 1961 Aug;10:555–565. doi: 10.1083/jcb.10.4.555. [DOI] [PMC free article] [PubMed] [Google Scholar]

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