Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1988 Jan;62(1):132–138. doi: 10.1128/jvi.62.1.132-138.1988

Molecular comparisons of in vivo- and in vitro-derived strains of Aleutian disease of mink parvovirus.

M E Bloom 1, O R Kaaden 1, E Huggans 1, A Cohn 1, J B Wolfinbarger 1
PMCID: PMC250511  PMID: 2824842

Abstract

DNA from one cell culture-adapted and two pathogenic strains of Aleutian disease of mink parvovirus (ADV) was molecularly cloned into the vectors pUC18 and pUC19. The DNA from the two pathogenic strains (ADV-Utah I and ADV-Pullman) was obtained from virus purified directly from the organs of infected mink, whereas the DNA from the nonpathogenic ADV-G was derived from cell culture material. The cloned segment from all three viruses represented a 3.55-kilobase-pair BamHI (15 map units) to HindIII (88 map units) fragment. Detailed physical mapping studies indicated that all three viruses shared 29 of 46 restriction endonuclease recognition sites but that 6 sites unique to the pathogenic strains and 5 sites unique to ADV-G were clustered in the portion of the genome expected to code for structural proteins. Clones from all three viruses directed the synthesis of two ADV-specific polypeptides with molecular weights of approximately 57 and 34 kilodaltons. Both species reacted with sera from infected mink as well as with a monoclonal antibody specific for ADV structural proteins. Because production of these ADV antigens was detected in both pUC18 and pUC19 and was not influenced by isopropyl-beta-D-thiogalactopyranoside (IPTG) induction, their expression was not regulated by the lac promoter of the pUC vector, but presumably by promoterlike sequences found within the ADV DNA. The proteins specified by the clones of ADV-G were 2 to 3 kilodaltons smaller than those of the two pathogenic strains, although the DNA segments were identical in size. This difference in protein molecular weights may correlate with pathogenicity, because capsid proteins of pathogenic and nonpathogenic strains of ADV exhibit a similar difference.

Full text

PDF
132

Images in this article

135Image
on p.135
135Image
on p.135
135Image
on p.135
136Image
on p.136

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aasted B. Aleutian disease of mink. Virology and immunology. Acta Pathol Microbiol Immunol Scand Suppl. 1985;287:1–47. [PubMed] [Google Scholar]
  2. Aasted B., Bloom M. E. Sensitive radioimmune assay for measuring Aleutian disease virus antigen and antibody. J Clin Microbiol. 1983 Sep;18(3):637–644. doi: 10.1128/jcm.18.3.637-644.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adhya S., Gottesman M. Control of transcription termination. Annu Rev Biochem. 1978;47:967–996. doi: 10.1146/annurev.bi.47.070178.004535. [DOI] [PubMed] [Google Scholar]
  4. Alexandersen S., Uttenthal-Jensen A., Aasted B. Demonstration of non-degraded Aleutian disease virus (ADV) proteins in lung tissue from experimentally infected mink kits. Brief report. Arch Virol. 1986;87(1-2):127–133. doi: 10.1007/BF01310549. [DOI] [PubMed] [Google Scholar]
  5. Astell C. R., Gardiner E. M., Tattersall P. DNA sequence of the lymphotropic variant of minute virus of mice, MVM(i), and comparison with the DNA sequence of the fibrotropic prototype strain. J Virol. 1986 Feb;57(2):656–669. doi: 10.1128/jvi.57.2.656-669.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Batteiger B., Newhall W. J., 5th, Jones R. B. The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. J Immunol Methods. 1982 Dec 30;55(3):297–307. doi: 10.1016/0022-1759(82)90089-8. [DOI] [PubMed] [Google Scholar]
  7. Ben-Asher E., Aloni Y. Transcription of minute virus of mice, an autonomous parvovirus, may be regulated by attenuation. J Virol. 1984 Oct;52(1):266–276. doi: 10.1128/jvi.52.1.266-276.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Berns K. I., Labow M. A. Parvovirus gene regulation. J Gen Virol. 1987 Mar;68(Pt 3):601–614. doi: 10.1099/0022-1317-68-3-601. [DOI] [PubMed] [Google Scholar]
  9. Bloom M. E., Lechner D., Wiedbrauk D. L., Wolfinbarger J. B. Analysis of molecularly cloned DNA reveals minor differences among three virus strains of Aleutian disease of mink parvovirus. Brief report. Arch Virol. 1987;92(1-2):175–181. doi: 10.1007/BF01310071. [DOI] [PubMed] [Google Scholar]
  10. Bloom M. E., Mayer L. W., Garon C. F. Characterization of the Aleutian disease virus genome and its intracellular forms. J Virol. 1983 Mar;45(3):977–984. doi: 10.1128/jvi.45.3.977-984.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Bloom M. E., Race R. E., Aasted B., Wolfinbarger J. B. Analysis of Aleutian disease virus infection in vitro and in vivo: demonstration of Aleutian disease virus DNA in tissues of infected mink. J Virol. 1985 Sep;55(3):696–703. doi: 10.1128/jvi.55.3.696-703.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Bloom M. E., Race R. E., Hadlow W. J., Chesebro B. Aleutian disease of mink: the antibody response of sapphire and pastel mink to Aleutian disease virus. J Immunol. 1975 Oct;115(4):1034–1037. [PubMed] [Google Scholar]
  13. Bloom M. E., Race R. E., Wolfinbarger J. B. Characterization of Aleutian disease virus as a parvovirus. J Virol. 1980 Sep;35(3):836–843. doi: 10.1128/jvi.35.3.836-843.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Bloom M. E., Race R. E., Wolfinbarger J. B. Identification of a nonvirion protein of Aleutian disease virus: mink with Aleutian disease have antibody to both virion and nonvirion proteins. J Virol. 1982 Aug;43(2):608–616. doi: 10.1128/jvi.43.2.608-616.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Chesebro B., Bloom M., Hadlow W., Race R. Purification and ultrastructure of Aleutian disease virus of mink. Nature. 1975 Apr 3;254(5499):456–457. doi: 10.1038/254456a0. [DOI] [PubMed] [Google Scholar]
  16. Eklund C. M., Hadlow W. J., Kennedy R. C., Boyle C. C., Jackson T. A. Aleutian disease of mink: properties of the etiologic agent and the host responses. J Infect Dis. 1968 Dec;118(5):510–526. doi: 10.1093/infdis/118.5.510. [DOI] [PubMed] [Google Scholar]
  17. Hadlow W. J., Race R. E., Kennedy R. C. Comparative pathogenicity of four strains of Aleutian disease virus for pastel and sapphire mink. Infect Immun. 1983 Sep;41(3):1016–1023. doi: 10.1128/iai.41.3.1016-1023.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hahn E. C., Ramos L., Kenyon A. J. Expression of Aleutian mink disease antigen in cell culture. Infect Immun. 1977 Jan;15(1):204–211. doi: 10.1128/iai.15.1.204-211.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  20. Ish-Horowicz D., Burke J. F. Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981 Jul 10;9(13):2989–2998. doi: 10.1093/nar/9.13.2989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Kaaden O. R., Haas L., Löchelt M., Roth S. Replication of Aleutian disease virus in mink lymphocytes infected in vitro. Intervirology. 1986;25(4):201–209. doi: 10.1159/000149676. [DOI] [PubMed] [Google Scholar]
  22. Kierek-Jaszczuk D., Moennig W., Stolze B., Neth R., Tan S., Greiser de Wilke I., Kaaden O. R. Epitopic mapping of structural and nonstructural Aleutian disease virus proteins. Intervirology. 1986;26(1-2):74–84. doi: 10.1159/000149684. [DOI] [PubMed] [Google Scholar]
  23. Kimsey P. B., Engers H. D., Hirt B., Jongeneel C. V. Pathogenicity of fibroblast- and lymphocyte-specific variants of minute virus of mice. J Virol. 1986 Jul;59(1):8–13. doi: 10.1128/jvi.59.1.8-13.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Kozak M. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles. Microbiol Rev. 1983 Mar;47(1):1–45. doi: 10.1128/mr.47.1.1-45.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Kozak M. Influences of mRNA secondary structure on initiation by eukaryotic ribosomes. Proc Natl Acad Sci U S A. 1986 May;83(9):2850–2854. doi: 10.1073/pnas.83.9.2850. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Löchelt M., Kaaden O. R. Lymphotropic strain SL3 of Aleutian disease virus: identification of replicative form DNA, molecular cloning and expression of capsid-specific proteins. J Gen Virol. 1987 Apr;68(Pt 4):1041–1048. doi: 10.1099/0022-1317-68-4-1041. [DOI] [PubMed] [Google Scholar]
  27. Mayer L. W., Aasted B., Garon C. F., Bloom M. E. Molecular cloning of the Aleutian disease virus genome: expression of Aleutian disease virus antigens by a recombinant plasmid. J Virol. 1983 Dec;48(3):573–579. doi: 10.1128/jvi.48.3.573-579.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Molitor T. W., Joo H. S., Collett M. S. KBSH parvovirus: comparison with porcine parvovirus. J Virol. 1985 Aug;55(2):257–263. doi: 10.1128/jvi.55.2.257-263.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Neve R. L., West R. W., Rodriguez R. L. Eukaryotic DNA fragments which act as promoters for a plasmid gene. Nature. 1979 Jan 25;277(5694):324–325. doi: 10.1038/277324a0. [DOI] [PubMed] [Google Scholar]
  30. Parrish C. R., Carmichael L. E. Characterization and recombination mapping of an antigenic and host range mutation of canine parvovirus. Virology. 1986 Jan 15;148(1):121–132. doi: 10.1016/0042-6822(86)90408-3. [DOI] [PubMed] [Google Scholar]
  31. Pintel D., Dadachanji D., Astell C. R., Ward D. C. The genome of minute virus of mice, an autonomous parvovirus, encodes two overlapping transcription units. Nucleic Acids Res. 1983 Feb 25;11(4):1019–1038. doi: 10.1093/nar/11.4.1019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Porter D. D. Aleutian disease: a persistent parvovirus infection of mink with a maximal but ineffective host humoral immune response. Prog Med Virol. 1986;33:42–60. [PubMed] [Google Scholar]
  33. Porter D. D., Larsen A. E., Cox N. A., Porter H. G., Suffin S. C. Isolation of Aleutian disease virus of mink in cell culture. Intervirology. 1977;8(3):129–144. doi: 10.1159/000148888. [DOI] [PubMed] [Google Scholar]
  34. Prakash O., Guntaka R. V., Sarkar N. H. Evidence for a prokaryotic promoter in the murine mammary tumor virus long terminal repeat. Gene. 1983 Aug;23(2):117–130. doi: 10.1016/0378-1119(83)90043-4. [DOI] [PubMed] [Google Scholar]
  35. Race R. E., Chesebro B., Bloom M. E., Aasted B., Wolfinbarger J. Monoclonal antibodies against Aleutian disease virus distinguish virus strains and differentiate sites of virus replication from sites of viral antigen sequestration. J Virol. 1986 Jan;57(1):285–293. doi: 10.1128/jvi.57.1.285-293.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Sahli R., McMaster G. K., Hirt B. DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice. Nucleic Acids Res. 1985 May 24;13(10):3617–3633. doi: 10.1093/nar/13.10.3617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Siegl G., Bates R. C., Berns K. I., Carter B. J., Kelly D. C., Kurstak E., Tattersall P. Characteristics and taxonomy of Parvoviridae. Intervirology. 1985;23(2):61–73. doi: 10.1159/000149587. [DOI] [PubMed] [Google Scholar]
  38. Smith H. O., Birnstiel M. L. A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976 Sep;3(9):2387–2398. doi: 10.1093/nar/3.9.2387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Tattersall P., Bratton J. Reciprocal productive and restrictive virus-cell interactions of immunosuppressive and prototype strains of minute virus of mice. J Virol. 1983 Jun;46(3):944–955. doi: 10.1128/jvi.46.3.944-955.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  42. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES