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. 1989 May;63(5):2340–2342. doi: 10.1128/jvi.63.5.2340-2342.1989

Mutational analysis of bovine papillomavirus E6 gene.

K H Vousden 1, E J Androphy 1, J T Schiller 1, D R Lowy 1
PMCID: PMC250653  PMID: 2539522

Abstract

The bovine papillomavirus E6 gene can independently transform mouse C127 cells. To characterize E6 in greater detail, we created 16 site-directed mutations in E6, including substitution mutations in the cysteine codons of the four Cys-X-X-Cys motifs that are conserved in all papillomavirus E6 proteins. Proteins mutated in six of the seven cysteines tested, as well as those lacking the nonconserved C-terminus, were stable in transfected cells but were unable to induce morphological transformation, indicating that these amino acids play an important role in the function of E6.

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Selected References

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