Abstract
The penetration of the CVS strain of rabies virus and its avirulent derivative AvO1 into peripheral neurons was investigated after intramuscular inoculation into the forelimbs of adult mice. It was found that CVS directly penetrates both the sensitive and motor routes with equal efficiency, without prior multiplication in muscle cells. Infected neurons became detectable 18 h after infection. The second cycle of infection occurred within 2 days, and at day 3 there was a massive invasion of the spinal cord and sensory ganglia. In sensory ganglia, where it was possible to identify cell outlines, it was evident that the infection did not proceed directly from cell body to cell body. The avirulent strain AvO1 penetrated motor and sensory neurons with the same efficiency as CVS. Restriction of viral propagation was observed from the second and third cycles onwards. No further development of the infection could be seen after day 3, and by that time the lysis of primarily infected neurons seemed to occur.
Full text
PDF




Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bulenga G., Heaney T. Post-exposure local treatment of mice infected with rabies with two axonal flow inhibitors, colchicine and vinblastine. J Gen Virol. 1978 May;39(2):381–385. doi: 10.1099/0022-1317-39-2-381. [DOI] [PubMed] [Google Scholar]
- Charlton K. M., Casey G. A. Experimental rabies in skunks: immunofluorescence light and electron microscopic studies. Lab Invest. 1979 Jul;41(1):36–44. [PubMed] [Google Scholar]
- Charlton K. M., Casey G. A. Experimental rabies in skunks: oral, nasal, tracheal and intestinal exposure. Can J Comp Med. 1979 Apr;43(2):168–172. [PMC free article] [PubMed] [Google Scholar]
- Coulon P., Rollin P., Aubert M., Flamand A. Molecular basis of rabies virus virulence. I. Selection of avirulent mutants of the CVS strain with anti-G monoclonal antibodies. J Gen Virol. 1982 Jul;61(Pt 50):97–100. doi: 10.1099/0022-1317-61-1-97. [DOI] [PubMed] [Google Scholar]
- Johnson R. T. Experimental rabies. Studies of cellular vulnerability and pathogenesis using fluorescent antibody staining. J Neuropathol Exp Neurol. 1965 Oct;24(4):662–674. [PubMed] [Google Scholar]
- Kucera P., Dolivo M., Coulon P., Flamand A. Pathways of the early propagation of virulent and avirulent rabies strains from the eye to the brain. J Virol. 1985 Jul;55(1):158–162. doi: 10.1128/jvi.55.1.158-162.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lycke E., Tsiang H. Rabies virus infection of cultured rat sensory neurons. J Virol. 1987 Sep;61(9):2733–2741. doi: 10.1128/jvi.61.9.2733-2741.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murphy F. A., Bauer S. P. Early street rabies virus infection in striated muscle and later progression to the central nervous system. Intervirology. 1974;3(4):256–268. doi: 10.1159/000149762. [DOI] [PubMed] [Google Scholar]
- Murphy F. A., Bauer S. P., Harrison A. K., Winn W. C., Jr Comparative pathogenesis of rabies and rabies-like viruses. Viral infection and transit from inoculation site to the central nervous system. Lab Invest. 1973 Mar;28(3):361–376. [PubMed] [Google Scholar]
- Murphy F. A. Rabies pathogenesis. Arch Virol. 1977;54(4):279–297. doi: 10.1007/BF01314774. [DOI] [PubMed] [Google Scholar]
- Prehaud C., Coulon P., LaFay F., Thiers C., Flamand A. Antigenic site II of the rabies virus glycoprotein: structure and role in viral virulence. J Virol. 1988 Jan;62(1):1–7. doi: 10.1128/jvi.62.1.1-7.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schneider L. G. Die Pathogenese der Tollwut bei der Maus. II. Die Virusaubreitung innerhalb des ZNS. Zentralbl Bakteriol Orig. 1969 Dec;212(1):1–13. [PubMed] [Google Scholar]
- Seif I., Coulon P., Rollin P. E., Flamand A. Rabies virulence: effect on pathogenicity and sequence characterization of rabies virus mutations affecting antigenic site III of the glycoprotein. J Virol. 1985 Mar;53(3):926–934. doi: 10.1128/jvi.53.3.926-934.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tsiang H. Evidence for an intraaxonal transport of fixed and street rabies virus. J Neuropathol Exp Neurol. 1979 May;38(3):286–299. doi: 10.1097/00005072-197905000-00008. [DOI] [PubMed] [Google Scholar]
- Watson H. D., Tignor G. H., Smith A. L. Entry of rabies virus into the peripheral nerves of mice. J Gen Virol. 1981 Oct;56(Pt 2):372–382. doi: 10.1099/0022-1317-56-2-371. [DOI] [PubMed] [Google Scholar]