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. 1989 Sep;63(9):4039–4042. doi: 10.1128/jvi.63.9.4039-4042.1989

Adenovirus type 12 E1A down regulates expression of a transgene under control of a major histocompatibility complex class I promoter: evidence for transcriptional control.

I Meijer 1, A G Jochemsen 1, C M de Wit 1, J L Bos 1, D Morello 1, A J van der Eb 1
PMCID: PMC251002  PMID: 2527313

Abstract

The E1 region of human adenovirus type 12 (Ad12 E1) represses the expression of major histocompatibility complex (MHC) class I genes in transformed primary rodent cells. Conflicting results have been reported as to whether this E1A-mediated repression occurs at a transcriptional or a posttranscriptional level. In the present study, we show that in Ad12 E1-transformed primary baby mouse kidney cells from transgenic mice harboring the human growth hormone gene under control of an H-2K promoter both the expression of the endogenous MHC class I genes and the expression of the transgene are repressed. This experiment, as well as nuclear run-on analyses performed with single-stranded probes, revealed that Ad12 E1A inhibits MHC class I gene expression by repressing its promoter.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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