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. 1989 Dec;63(12):5159–5165. doi: 10.1128/jvi.63.12.5159-5165.1989

The translational requirement for complete La Crosse virus mRNA synthesis is cell-type dependent.

R Raju 1, L Raju 1, D Kolakofsky 1
PMCID: PMC251179  PMID: 2573737

Abstract

The translational requirement to prevent premature termination during La Crosse virus S mRNA synthesis was found to be cell-type dependent. This requirement was present in the BHK, HEL, and Vero cell lines we examined, but not in C6/36 mosquito cells. The cell-dependent translational requirement could be reproduced in vitro by using either cell extracts or purified virions of BHK and C6/36 cells. In the BHK reactions, the polymerase terminated predominantly at nucleotide 175 in the absence of concurrent translation and required translation to read through this position. In the C6/36 reactions, however, the polymerase reads through nucleotide 175 efficiently independent of translation. Reconstitution studies suggested that the translational requirement was due to a factor(s) present in BHK, but not in C6/36, cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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