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. 1989 Dec;63(12):5194–5200. doi: 10.1128/jvi.63.12.5194-5200.1989

Viral DNA in horses infected with equine infectious anemia virus.

N R Rice 1, A S Lequarre 1, J W Casey 1, S Lahn 1, R M Stephens 1, J Edwards 1
PMCID: PMC251183  PMID: 2555550

Abstract

The amount and distribution of viral DNA were established in a horse acutely infected with the Wyoming strain of equine infectious anemia virus (EIAV). The highest concentration of viral DNA were found in the liver, lymph nodes, bone marrow, and spleen. The kidney, choroid plexus, and peripheral blood leukocytes also contained viral DNA, but at a lower level. It is estimated that at day 16 postinoculation, almost all of the viral DNA was located in the tissues, with the liver alone containing about 90 times more EIAV DNA than the peripheral blood leukocytes did. Assuming a monocyte-macrophage target, each infected cell contained multiple copies of viral DNA (between 6 and 60 copies in liver Kupffer cells). At day 16 postinoculation, most of the EIAV DNA was not integrated into host DNA, but existed in both linear and circular unintegrated forms. In contrast to acute infection, viral DNA was not detectable in tissues from asymptomatic horses with circulating antibody to EIAV.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benton C. V., Brown B. L., Harshman J. S., Gilden R. V. In vitro host range of equine infectious anemia virus. Intervirology. 1981;16(4):225–232. doi: 10.1159/000149271. [DOI] [PubMed] [Google Scholar]
  2. Blouin A., Bolender R. P., Weibel E. R. Distribution of organelles and membranes between hepatocytes and nonhepatocytes in the rat liver parenchyma. A stereological study. J Cell Biol. 1977 Feb;72(2):441–455. doi: 10.1083/jcb.72.2.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cheevers W. P., McGuire T. C. Equine infectious anemia virus: immunopathogenesis and persistence. Rev Infect Dis. 1985 Jan-Feb;7(1):83–88. doi: 10.1093/clinids/7.1.83. [DOI] [PubMed] [Google Scholar]
  4. Chiu I. M., Yaniv A., Dahlberg J. E., Gazit A., Skuntz S. F., Tronick S. R., Aaronson S. A. Nucleotide sequence evidence for relationship of AIDS retrovirus to lentiviruses. 1985 Sep 26-Oct 2Nature. 317(6035):366–368. doi: 10.1038/317366a0. [DOI] [PubMed] [Google Scholar]
  5. Clements J. E., Gdovin S. L., Montelaro R. C., Narayan O. Antigenic variation in lentiviral diseases. Annu Rev Immunol. 1988;6:139–159. doi: 10.1146/annurev.iy.06.040188.001035. [DOI] [PubMed] [Google Scholar]
  6. Coggins L., Norcross N. L., Nusbaum S. R. Diagnosis of equine infectious anemia by immunodiffusion test. Am J Vet Res. 1972 Jan;33(1):11–18. [PubMed] [Google Scholar]
  7. Evans K. S., Carpenter S. L., Sevoian M. Detection of equine infectious anemia virus in horse leukocyte cultures derived from horses in various stages of equine infectious anemia viral infection. Am J Vet Res. 1984 Jan;45(1):20–25. [PubMed] [Google Scholar]
  8. Fisher A. G., Ensoli B., Looney D., Rose A., Gallo R. C., Saag M. S., Shaw G. M., Hahn B. H., Wong-Staal F. Biologically diverse molecular variants within a single HIV-1 isolate. Nature. 1988 Aug 4;334(6181):444–447. doi: 10.1038/334444a0. [DOI] [PubMed] [Google Scholar]
  9. Henderson L. E., Sowder R. C., Smythers G. W., Oroszlan S. Chemical and immunological characterizations of equine infectious anemia virus gag-encoded proteins. J Virol. 1987 Apr;61(4):1116–1124. doi: 10.1128/jvi.61.4.1116-1124.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Issel C. J., Adams W. V., Jr, Meek L., Ochoa R. Transmission of equine infectious anemia virus from horses without clinical signs of disease. J Am Vet Med Assoc. 1982 Feb 1;180(3):272–275. [PubMed] [Google Scholar]
  11. Kawakami T., Sherman L., Dahlberg J., Gazit A., Yaniv A., Tronick S. R., Aaronson S. A. Nucleotide sequence analysis of equine infectious anemia virus proviral DNA. Virology. 1987 Jun;158(2):300–312. doi: 10.1016/0042-6822(87)90202-9. [DOI] [PubMed] [Google Scholar]
  12. Kobayashi K., Kono Y. Propagation and titration of equine infectious anemia virus in horse leukocyte culture. Natl Inst Anim Health Q (Tokyo) 1967 Spring;7(1):8–20. [PubMed] [Google Scholar]
  13. Kobayashi K., Kono Y. Serial passages of equine infectious anemia virus in horse leukocyte culture. Natl Inst Anim Health Q (Tokyo) 1967 Spring;7(1):1–7. [PubMed] [Google Scholar]
  14. Kono Y. Viremia and immunological responses in horses infected with equine infectious anemia virus. Natl Inst Anim Health Q (Tokyo) 1969 Spring;9(1):1–9. [PubMed] [Google Scholar]
  15. Kono Y., Yokomizo Y. Attempts to cultivate the equine infectious anemia virus in various types of cells. Natl Inst Anim Health Q (Tokyo) 1968 Winter;8(4):182–186. [PubMed] [Google Scholar]
  16. Kono Y., Yoshino T. Propagation of equine infectious anemia virus in horse kidney cell cultures. Natl Inst Anim Health Q (Tokyo) 1974 Winter;14(4):155–162. [PubMed] [Google Scholar]
  17. McConnel M. B., Katada M., McConnell S., Moore R. Demonstration of equine infectious anemia virus in primary leukocyte cultures by electron microscopy. Am J Vet Res. 1977 Dec;38(12):2067–2069. [PubMed] [Google Scholar]
  18. McGuire T. C., Crawford T. B., Henson J. B. Immunofluorescent localization of equine infectious anemia virus in tissue. Am J Pathol. 1971 Feb;62(2):283–294. [PMC free article] [PubMed] [Google Scholar]
  19. O'Rourke K., Perryman L. E., McGuire T. C. Antiviral, anti-glycoprotein and neutralizing antibodies in foals with equine infectious anaemia virus. J Gen Virol. 1988 Mar;69(Pt 3):667–674. doi: 10.1099/0022-1317-69-3-667. [DOI] [PubMed] [Google Scholar]
  20. Rushlow K., Olsen K., Stiegler G., Payne S. L., Montelaro R. C., Issel C. J. Lentivirus genomic organization: the complete nucleotide sequence of the env gene region of equine infectious anemia virus. Virology. 1986 Dec;155(2):309–321. doi: 10.1016/0042-6822(86)90195-9. [DOI] [PubMed] [Google Scholar]
  21. Stephens R. M., Casey J. W., Rice N. R. Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus. Science. 1986 Feb 7;231(4738):589–594. doi: 10.1126/science.3003905. [DOI] [PubMed] [Google Scholar]
  22. Tajima M., Nakajima H., Ito Y. Electron microscopy of equine infectious anemia virus. J Virol. 1969 Oct;4(4):521–527. doi: 10.1128/jvi.4.4.521-527.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ushimi C., Henson J. B., Gorham J. R. Study of the one-step growth curve of equine infectious anemia virus by immunofluorescence. Infect Immun. 1972 Jun;5(6):890–895. doi: 10.1128/iai.5.6.890-895.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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