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. 1972 Jul;111(1):73–79. doi: 10.1128/jb.111.1.73-79.1972

Mapping of rod Mutants of Bacillus subtilis

D Karamata 1, M McConnell 1, H J Rogers 1
PMCID: PMC251241  PMID: 4204911

Abstract

Nine class A salt-dependent rod mutants were mapped on the Bacillus subtilis genome by PBS1-mediated transduction. They are distributed into two small linkage groups designated rod B and rod C; mutations in rod B are over 80% cotransducible with pheA and different mutations in rod C are 12 to 21% cotransducible with hisA. It is established that neither rod B nor rod C is linked by transformation to the other identified rod mutations present in 168-ts-200B and 8332 glu−. It is hypothesized that salt-dependent mutations are due to enzyme alterations which are corrected by high salt concentrations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boylan R. J., Mendelson N. H. Initial characterization of a temperature-sensitive rod--mutant of Bacillus subtilis. J Bacteriol. 1969 Dec;100(3):1316–1321. doi: 10.1128/jb.100.3.1316-1321.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carlton B. C. Fine-structure mapping by transformation in the tryptophan region of Bacillus subtilis. J Bacteriol. 1966 May;91(5):1795–1803. doi: 10.1128/jb.91.5.1795-1803.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cerdá-Olmedo E., Hanawalt P. C. The replication of the Escherichia coli chromosome studied by sequential nitrosoguanidine mutagenesis. Cold Spring Harb Symp Quant Biol. 1968;33:599–607. doi: 10.1101/sqb.1968.033.01.066. [DOI] [PubMed] [Google Scholar]
  4. Cole R. M., Popkin T. J., Boylan R. J., Mendelson N. H. Ultrastructure of a temperature-sensitive rod- mutant of Bacillus subtilis. J Bacteriol. 1970 Sep;103(3):793–810. doi: 10.1128/jb.103.3.793-810.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dubnau D., Goldthwaite C., Smith I., Marmur J. Genetic mapping in Bacillus subtilis. J Mol Biol. 1967 Jul 14;27(1):163–185. doi: 10.1016/0022-2836(67)90358-0. [DOI] [PubMed] [Google Scholar]
  6. Grant G. F., Simon M. I. Synthesis of bacterial flagella. II. PBS1 transduction of flagella-specific markers in Bacillus subtilis. J Bacteriol. 1969 Jul;99(1):116–124. doi: 10.1128/jb.99.1.116-124.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Karamata D., Gross J. D. Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Mol Gen Genet. 1970;108(3):277–287. doi: 10.1007/BF00283358. [DOI] [PubMed] [Google Scholar]
  8. Rogers H. J., McConnell M., Burdett I. D. Cell wall or membrane mutants of Bacillus subtilis and Bacillus licheniformis with grossly deformed morphology. Nature. 1968 Jul 20;219(5151):285–288. doi: 10.1038/219285a0. [DOI] [PubMed] [Google Scholar]
  9. Rogers H. J., McConnell M., Burdett I. D. The isolation and characterization of mutants of Bacillus subtilis and Bacillus licheniformis with disturbed morphology and cell division. J Gen Microbiol. 1970 May;61(2):155–171. doi: 10.1099/00221287-61-2-155. [DOI] [PubMed] [Google Scholar]
  10. Rogers H. J., McConnell M., Hughes R. C. The chemistry of the cell walls of rod mutants of Bacillus subtilis. J Gen Microbiol. 1971 Jun;66(3):297–308. doi: 10.1099/00221287-66-3-297. [DOI] [PubMed] [Google Scholar]
  11. Rogers H. J., McConnell M. The role of L-glutamine in the phenotypic change of a rod mutant derived from Bacillus subtilis 168. J Gen Microbiol. 1970 May;61(2):173–181. doi: 10.1099/00221287-61-2-173. [DOI] [PubMed] [Google Scholar]
  12. Siccardi A. G., Shapiro B. M. On the process of cellular division in Escherichia coli. IV. Altered protein composition and turnover of the membranes of thermosensitive mutants defective in chromosomal replication. J Mol Biol. 1971 Mar 28;56(3):475–490. doi: 10.1016/0022-2836(71)90395-0. [DOI] [PubMed] [Google Scholar]
  13. YOSHIKAWA H., SUEOKA N. Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phases. Proc Natl Acad Sci U S A. 1963 Apr;49:559–566. doi: 10.1073/pnas.49.4.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Young F. E., Smith C., Reilly B. E. Chromosomal location of genes regulating resistance to bacteriophage in Bacillus subtilis. J Bacteriol. 1969 Jun;98(3):1087–1097. doi: 10.1128/jb.98.3.1087-1097.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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