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. 1972 Jul;111(1):112–118. doi: 10.1128/jb.111.1.112-118.1972

Documentation of Auxotrophic Mutation in Blue-Green Bacteria: Characterization of a Tryptophan Auxotroph in Agmenellum quadruplicatum

Lonnie O Ingram a, Duane Pierson a, James F Kane a,1, C Van Baalen a, Roy A Jensen a
PMCID: PMC251247  PMID: 4204902

Abstract

A tryptophan-requiring auxotroph of Agmenellum quadruplicatum strain BG1, a species of blue-green bacteria, was isolated by means of a nitrosoguanidine-penicillin procedure. Its growth characteristics were determined, and the enzymological block was identified in the A activity of tryptophan synthetase. Starvation of the auxotroph for tryptophan resulted in the derepression of the synthesis of all five enzymes. The first four enzymes derepressed 2- to 3-fold, and tryptophan synthetase B derepressed 20-fold. In the parental prototroph, BG1, anthranilate synthetase was active in crude extracts with ammonia as the amino donor reactant, but not with glutamine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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