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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Jul;111(1):119–128. doi: 10.1128/jb.111.1.119-128.1972

Abnormal Excision and Transfer of Chromosomal Segments by a Strain of Escherichia coli K-12

Peter L Bergquist 1, Edward A Adelberg 2
PMCID: PMC251248  PMID: 4591470

Abstract

PB15 is an Hfr strain of Escherichia coli K-12. It arose from an F′ strain carrying a temperature-sensitive F-gal by an event which blocked the detachment of F-gal in the normally reversible integration process. In PB15, the detachment of F-gal by a second mechanism can now be detected: this mechanism results in the excision and transfer of extended chromosomal segments which include the integrated F-gal; the excised segments are inferred to have circularized. Their excision, which is independent of the recA+ allele, occurs at an unusually high rate during conjugation; a mutant F-initiator protein is suggested as the cause of this phenomenon. After their establishment in recipients, the enlarged F-genotes undergo further deletions of included donor genes by a process which is again recA+-independent. In Rec+, but not in Rec, cells, a high proportion of the deleted fragments are rescued by integration into the recipient's chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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