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. 1972 Jul;111(1):156–162. doi: 10.1128/jb.111.1.156-162.1972

Deoxyribonucleic Acid Distribution in Bacillus subtilis Independent of Cell Elongation

Neil H Mendelson 1
PMCID: PMC251252  PMID: 4204904

Abstract

A temperature-sensitive DNA− mutant of Bacillus subtilis has been studied during the resumption of deoxyribonucleic acid (DNA) synthesis following a 45 to 30 C temperature shift. For several hours after return to 30 C, DNA synthesis proceeds although the cells fail to elongate appreciably. Autoradiographs of cell populations synthesizing DNA during the recovery period demonstrate that DNA can become distributed to previously unoccupied regions along the cell length. By varying the labeling regime, newly synthesized DNA as well as DNA present at the time of transfer from 45 to 30 C were followed independently. Measurements of the percent of cell length covered by grains (3H-thymine in DNA) demonstrate the progressive refilling of DNA-vacant cell regions by both newly synthesized and original DNA. These data indicate that cell surface growth is not an absolute requirement for segregation of bacterial DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison D. P. Giant cells of Escherichia coli: a morphological study. J Bacteriol. 1971 Dec;108(3):1390–1401. doi: 10.1128/jb.108.3.1390-1401.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boylan R. J., Mendelson N. H. Initial characterization of a temperature-sensitive rod--mutant of Bacillus subtilis. J Bacteriol. 1969 Dec;100(3):1316–1321. doi: 10.1128/jb.100.3.1316-1321.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CARO L. G., VAN TUBERGEN R. P., KOLB J. A. High-resolution autoradiography. I. Methods. J Cell Biol. 1962 Nov;15:173–188. doi: 10.1083/jcb.15.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Caro L. G. Chromosome replication in Escherichia coli. 3. Segregation of chromosomal strands in multi-forked replication. J Mol Biol. 1970 Mar 14;48(2):329–338. doi: 10.1016/0022-2836(70)90164-6. [DOI] [PubMed] [Google Scholar]
  5. Caro L. G., Schnös M. Tritium and Phosphorus-32 in High-Resolution Autoradiography. Science. 1965 Jul 2;149(3679):60–62. doi: 10.1126/science.149.3679.60. [DOI] [PubMed] [Google Scholar]
  6. Chai N. C., Lark K. G. Cytological studies of deoxyribonucleic acid replication in Escherichia coli 15T-: replication at slow growth rates and after a shift-up into rich medium. J Bacteriol. 1970 Oct;104(1):401–409. doi: 10.1128/jb.104.1.401-409.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cole R. M. Symposium on the fine structure and replication of bacteria and their parts. 3. Bacterial cell-wall replication followed by immunofluorescence. Bacteriol Rev. 1965 Sep;29(3):326–344. doi: 10.1128/br.29.3.326-344.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Donachie W. D., Begg K. J. Growth of the bacterial cell. Nature. 1970 Sep 19;227(5264):1220–1224. doi: 10.1038/2271220a0. [DOI] [PubMed] [Google Scholar]
  9. Gross J. D., Karamata D., Hempstead P. G. Temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Cold Spring Harb Symp Quant Biol. 1968;33:307–312. doi: 10.1101/sqb.1968.033.01.034. [DOI] [PubMed] [Google Scholar]
  10. Ivarie R. D., Pène J. J. Association of the Bacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients. J Bacteriol. 1970 Nov;104(2):839–850. doi: 10.1128/jb.104.2.839-850.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jacob F., Ryter A., Cuzin F. On the association between DNA and membrane in bacteria. Proc R Soc Lond B Biol Sci. 1966 Mar 22;164(995):267–278. doi: 10.1098/rspb.1966.0029. [DOI] [PubMed] [Google Scholar]
  12. Karamata D., Gross J. D. Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Mol Gen Genet. 1970;108(3):277–287. doi: 10.1007/BF00283358. [DOI] [PubMed] [Google Scholar]
  13. Lin E. C., Hirota Y., Jacob F. On the process of cellular division in Escherichia coli. VI. Use of a methocel-autoradiographic method for the study of cellular division in Escherichia coli. J Bacteriol. 1971 Oct;108(1):375–385. doi: 10.1128/jb.108.1.375-385.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. MASON D. J., POWELSON D. M. Nuclear division as observed in live bacteria by a new technique. J Bacteriol. 1956 Apr;71(4):474–479. doi: 10.1128/jb.71.4.474-479.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mendelson N. H. Can defective segregation prevent initiation? Cold Spring Harb Symp Quant Biol. 1968;33:313–316. doi: 10.1101/sqb.1968.033.01.035. [DOI] [PubMed] [Google Scholar]
  16. Mendelson N. H. Nuclear segregation without DNA replication in Bacillus subtilis. Biochim Biophys Acta. 1969 Sep 17;190(1):132–138. doi: 10.1016/0005-2787(69)90162-2. [DOI] [PubMed] [Google Scholar]
  17. Reeve J. N., Groves D. J., Clark D. J. Regulation of Cell Division in Escherichia coli: Characterization of Temperature-Sensitive Division Mutants. J Bacteriol. 1970 Dec;104(3):1052–1064. doi: 10.1128/jb.104.3.1052-1064.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rogers H. J. Bacterial growth and the cell envelope. Bacteriol Rev. 1970 Jun;34(2):194–214. doi: 10.1128/br.34.2.194-214.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ryter A. Association of the nucleus and the membrane of bacteria: a morphological study. Bacteriol Rev. 1968 Mar;32(1):39–54. doi: 10.1128/br.32.1.39-54.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Ryter A. Etude de la croissance de la membrane chez Bacillus subtilis au moyen de la distribution des flagelles. Ann Inst Pasteur (Paris) 1971 Sep;121(3):271–288. [PubMed] [Google Scholar]
  21. Ryter A., Hirota Y., Jacob F. DNA-membrane complex and nuclear segregation in bacteria. Cold Spring Harb Symp Quant Biol. 1968;33:669–676. doi: 10.1101/sqb.1968.033.01.076. [DOI] [PubMed] [Google Scholar]
  22. Ryter A. Relationship between synthesis of the cytoplasmic membrane and nuclear segregation in Bacillus subtilis. Folia Microbiol (Praha) 1967;12(3):283–290. doi: 10.1007/BF02868745. [DOI] [PubMed] [Google Scholar]
  23. SCHAECHTER M., LAING V. O. Direct observation of fusion of bacterial nuclei. J Bacteriol. 1961 Apr;81:667–668. doi: 10.1128/jb.81.4.667-668.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tremblay G. Y., Daniels M. J., Schaechter M. Isolation of a cell membrane-DNA-nascent RNA complex from bacteria. J Mol Biol. 1969 Feb 28;40(1):65–76. doi: 10.1016/0022-2836(69)90296-4. [DOI] [PubMed] [Google Scholar]

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