Abstract
A rapid method of determining the rate of transformation of group H streptococci to streptomycin resistance has been developed. The new technique, which involves analysis of the growth response of transformed streptococci in liquid medium containing streptomycin, is independent of chain length fluctuations and is demonstrably more accurate than the standard plating method. The relatively short generation time of streptococci under these conditions permits transformation rates to be estimated in 9 to 14 hr depending on the number of transformants in the inocula as compared to 50 hr by the agar plate procedure.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Herrington B. L. A Note Regarding the Lag Period. J Bacteriol. 1934 Aug;28(2):177–179. doi: 10.1128/jb.28.2.177-179.1934. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ingraham M. A. The Bacteriostatic Action of Gentian Violet and Dependence on the Oxidation-Reduction Potential. J Bacteriol. 1933 Dec;26(6):573–598. doi: 10.1128/jb.26.6.573-598.1933. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- PERRY D., SLADE H. D. INTRASPECIFIC AND INTERSPECIFIC TRANSFORMATION IN STREPTOCOCCI. J Bacteriol. 1964 Sep;88:595–601. doi: 10.1128/jb.88.3.595-601.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perry D., Slade H. D. Effect of filtrates from transformable and nontransformable streptococci on the transformation of streptococci. J Bacteriol. 1966 Jun;91(6):2216–2222. doi: 10.1128/jb.91.6.2216-2222.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SAGERS R. D. An efficient, easily constructed cell homogenizing press. Appl Microbiol. 1962 Jan;10:37–39. doi: 10.1128/am.10.1.37-39.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YOUNG F. E., SPIZIZEN J. Physiological and genetic factors affecting transformation of Bacillus subtilis. J Bacteriol. 1961 May;81:823–829. doi: 10.1128/jb.81.5.823-829.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]