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. 1972 Aug;111(2):375–382. doi: 10.1128/jb.111.2.375-382.1972

Mutants of Salmonella typhimurium That Are Insensitive to Catabolite Repression of Proline Degradation

Sara Lee Newell 1, Winston J Brill 1
PMCID: PMC251293  PMID: 4559730

Abstract

In Salmonella typhimurium the two enzymes of proline catabolism, proline oxidase and Δ1-pyrroline-5-carboxylic acid dehydrogenase, are subject to catabolite repression when the cells are grown in the presence of glucose. Mutants partially relieved of catabolite repression (PutR) for the proline catabolic enzymes have been isolated by selection on agar plates containing glucose and proline. The specificity of the catabolite repression-insensitive character for the enzymes of proline utilization has been confirmed by an analysis of other unrelated catabolic enzymes. Histidase and amylomaltase of the mutant strains are equally as sensitive to glucose repression as are the enzymes from the wild type. All four PutR mutants exhibit higher induced and higher basal levels of proline oxidase as compared with the corresponding wild-type levels. The mutations of three strains tested are cotransducible with constitutive, pleiotrophic-negative and structural gene mutations of the put region. Three-factor crosses indicate that two putR mutations are located at one end of the cluster of put mutations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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