Abstract
The uptake of 14C-α-methyl-d-glucoside (αMG) by washed cells of Mycoplasma strain Y was found to be dependent on the supply of metabolic energy. Glycerol or d-mannose, but not l-lactate, would serve as an energy source. Uptake was inhibited by fluoride, iodoacetate, and arsenate, but not by 2,4-dinitrophenol. d-Glucose was inhibitory, presumably by competing for the transport system. The initial product of accumulation had the properties of a phosphate ester of αMG. The proportion of free αMG in the cells increased with time, until a steady state was reached in which uptake was balanced by the efflux of free αMG from the cells. Broken-cell preparations catalyzed a phosphoenolpyruvate-dependent phosphorylation of αMG and of d-glucose.
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Selected References
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