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. 1972 Aug;111(2):465–473. doi: 10.1128/jb.111.2.465-473.1972

Inducible Uptake System for β-Carboxy-cis, cis-muconate in a Permeability Mutant of Pseudomonas putida

Richard B Meagher 1, George M McCorkle 1, M K Ornston 1, L N Ornston 1
PMCID: PMC251306  PMID: 5053469

Abstract

A procedure for the large-scale enzymatic synthesis of β-carboxymuconate is described. When used as a growth substrate, β-carboxymuconate selected for mutant strains of Pseudomonas putida that were permeable to polycarboxylic acid intermediates of the β-ketoadipate pathway. One mutant organism, strain PRS2110, was investigated in detail. It differed from the parental strain in that it possessed a β-carboxymuconate uptake system that was formed when the compound was supplied exogenously to the cells. The uptake system was not induced by β-carboxymuconate supplied endogenously during growth with p-hydroxybenzoate. These observations suggested that β-carboxymuconate was contained within a physical compartment of enzymes during growth with p-hydroxybenzoate. Support for this hypothesis came from the demonstration that enzymes of the β-ketoadipate pathway were held together by weak chemical interactions during the chromatography of crude extracts of benzoategrown P. putida on diethylaminoethyl-cellulose columns.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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