Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Aug;111(2):575–585. doi: 10.1128/jb.111.2.575-585.1972

Requirement for Protein Synthesis in rec-Dependent Repair of Deoxyribonucleic Acid in Escherichia coli after Ultraviolet or X Irradiation

Ann K Ganesan a,1, Kendric C Smith a
PMCID: PMC251320  PMID: 4559738

Abstract

Deprivation of amino acids required for growth or treatment with chloramphenicol or puromycin after irradiation reduced the survival of Rec+ cells of Escherichia coli K-12 which had been exposed to either ultraviolet (UV) or X radiation. In contrast, these treatments caused little or no reduction in the survival of irradiated recA or recB mutants. The effect of chloramphenicol on the survival of X-irradiated cells was correlated with an inhibition of repair of single-strand breaks in irradiated deoxyribonucleic acid (DNA), previously shown to be controlled by recA and recB. In UV-irradiated cells no effect of chloramphenicol was detected on the repair of single-strand discontinuities in DNA replicated from UV-damaged templates, a process controlled by recA but not by recB. From this we concluded that inhibiting protein synthesis in UV or X-irradiated cells may interfere with some biochemical step in repair dependent upon the recB gene. When irradiated Rec+ cells were cultured for a sufficient period of time in minimal growth medium before chloramphenicol treatment their survival was no longer decreased by the drug. After X irradiation this occurred in less than one generation time of the unirradiated control cells. After UV irradiation it occurred more slowly and was only complete after several generation times of the unirradiated controls. These observations indicated that replication of the entire irradiated genome was probably not required for rec-dependent repair of X-irradiated cells, although it might be required for rec-dependent repair of UV-irradiated cells.

Full text

PDF
575

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALPER T. LETHAL MUTATIONS AND CELL DEATH. Phys Med Biol. 1963 Nov;8:365–385. doi: 10.1088/0031-9155/8/4/301. [DOI] [PubMed] [Google Scholar]
  2. BILLEN D., JORGENSEN G. N. ENVIRONMENTAL CONDITIONS AFFECTING THE EXPRESSION OF X-RAY-INDUCED LETHALITY IN AN AUXOTROPHIC BACTERIUM. Radiat Res. 1964 Jul;22:535–542. [PubMed] [Google Scholar]
  3. Clark A. J. Toward a metabolic interpretation of genetic recombination of E. coli and its phages. Annu Rev Microbiol. 1971;25:437–464. doi: 10.1146/annurev.mi.25.100171.002253. [DOI] [PubMed] [Google Scholar]
  4. Curtiss R., 3rd Ultraviolet-induced genetic recombination in a partially diploid strain of Escherichia coli. Genetics. 1968 Jan;58(1):9–54. doi: 10.1093/genetics/58.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  6. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fangman W. L., Russel M. X-irradiation sensitivity in Escherichia coli defective in DNA replication. Mol Gen Genet. 1971;110(4):332–347. doi: 10.1007/BF00438275. [DOI] [PubMed] [Google Scholar]
  8. Forage A. J., Gillies N. E. Enhancement of damage to Escherichia coli strain B-R after ultraviolet and gamma irradiation. J Gen Microbiol. 1969 Dec;59(3):329–336. doi: 10.1099/00221287-59-3-329. [DOI] [PubMed] [Google Scholar]
  9. Fuks Z., Smith K. C. Effect of quinacrine on x-ray sensitivity and the repair of damaged DNA in Escherichia coli K-12. Radiat Res. 1971 Oct;48(1):63–73. [PubMed] [Google Scholar]
  10. Ganesan A. K., Smith K. C. Dark recovery processes in Escherichia coli irradiated with ultraviolet light. I. Effect of rec mutations on liquid holding recovery. J Bacteriol. 1968 Aug;96(2):365–373. doi: 10.1128/jb.96.2.365-373.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ganesan A. K., Smith K. C. Dark-recovery processes in Escherichia coli irradiated with ultraviolet light. 3. Effect of rec mutations on recovery of excision-deficient mutants of Escherichia coli K-12. J Bacteriol. 1970 May;102(2):404–410. doi: 10.1128/jb.102.2.404-410.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ganesan A. K., Smith K. C. The duration of recovery and DNA repair in excision deficient derivatives of Escherichia coli K-12 after ultraviolet irradiation. Mol Gen Genet. 1971;113(4):285–296. doi: 10.1007/BF00272328. [DOI] [PubMed] [Google Scholar]
  13. Geisselsoder J., Karzmark C. J. G-value determination of the Fricke dosemeter for 50 kVp x-rays. Phys Med Biol. 1969 Jan;14(1):61–71. doi: 10.1088/0031-9155/14/1/308. [DOI] [PubMed] [Google Scholar]
  14. Gillies N. E., Brown D. A possible explanation of chloramphenicol death in irradiated Escherichia coli b/r. Biochem Biophys Res Commun. 1967 Jan 10;26(1):102–107. doi: 10.1016/0006-291x(67)90259-8. [DOI] [PubMed] [Google Scholar]
  15. Gross J., Gross M. Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1166–1168. doi: 10.1038/2241166a0. [DOI] [PubMed] [Google Scholar]
  16. Howard-Flanders P., Boyce R. P., Theriot L. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics. 1966 Jun;53(6):1119–1136. doi: 10.1093/genetics/53.6.1119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kapp D. S., Smith K. C. Repair of radiation-induced damage in Escherichia coli. II. Effect of rec and uvr mutations on radiosensitivity, and repair of x-ray-induced single-strand breaks in deoxyribonucleic acid. J Bacteriol. 1970 Jul;103(1):49–54. doi: 10.1128/jb.103.1.49-54.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kapp D. S., Smith K. C. The chemical inhibition of the repair of single-strand breaks in DNA: post-irradiation sensitization to x-rays. Int J Radiat Biol Relat Stud Phys Chem Med. 1971;19(3):255–262. doi: 10.1080/09553007114550361. [DOI] [PubMed] [Google Scholar]
  19. LOEVINGER R., HUISMAN P. A TWIN-TUBE 50-KVP BERYLLIUM-WINDOW X-RAY UNIT FOR MICROBIAL RADIOBIOLOGY. Radiat Res. 1965 Feb;24:357–367. [PubMed] [Google Scholar]
  20. Marshall N. J., Gillies N. E. Chloramphenicol rescue of -irradiated lon and exrA mutants of Escherichia coli. Mutat Res. 1972 Jan;14(1):13–21. doi: 10.1016/0027-5107(72)90103-0. [DOI] [PubMed] [Google Scholar]
  21. Monk M., Peacey M., Gross J. D. Repair of damage induced by ultraviolet light in DNA polymerase-defective Escherichia coli cells. J Mol Biol. 1971 Jun 14;58(2):623–630. doi: 10.1016/0022-2836(71)90376-7. [DOI] [PubMed] [Google Scholar]
  22. OKAGAKI H. Effect of chloramphenicol on the survival of Escherichia coli irradiated with ultraviolet light. J Bacteriol. 1960 Feb;79:277–291. doi: 10.1128/jb.79.2.277-291.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rupp W. D., Wilde C. E., 3rd, Reno D. L., Howard-Flanders P. Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli. J Mol Biol. 1971 Oct 14;61(1):25–44. doi: 10.1016/0022-2836(71)90204-x. [DOI] [PubMed] [Google Scholar]
  24. Smith K. C., Meun D. H. Repair of radiation-induced damage in Escherichia coli. I. Effect of rec mutations on post-replication repair of damage due to ultraviolet radiation. J Mol Biol. 1970 Aug;51(3):459–472. doi: 10.1016/0022-2836(70)90001-x. [DOI] [PubMed] [Google Scholar]
  25. Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Town C. D., Smith K. C., Kaplan H. S. Production and repair of radiochemical damage in Escherichia coli deoxyribonucleic acid; its modification by culture conditions and relation to survival. J Bacteriol. 1971 Jan;105(1):127–135. doi: 10.1128/jb.105.1.127-135.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Willetts N. S., Clark A. J. Characteristics of some multiply recombination-deficient strains of Escherichia coli. J Bacteriol. 1969 Oct;100(1):231–239. doi: 10.1128/jb.100.1.231-239.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES