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. 1972 Sep;111(3):821–824. doi: 10.1128/jb.111.3.821-824.1972

Yeast Spheroplasts Formed by Cell Wall-Degrading Enzymes from Oerskovia sp1

J W Mann a, C E Heintz a, J D Macmillan a
PMCID: PMC251358  PMID: 4559832

Abstract

Oerskovia sp. produces inducible extracellular enzymes which degrade the walls of various yeasts. Yeast spheroplasts are formed from both log- and stationary-phase cells.

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Selected References

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  1. Anderson F. B., Millbank J. W. Protoplast formation and yeast cell-wall structure. The action of the enzymes of the snail, Helix pomatia. Biochem J. 1966 Jun;99(3):682–687. doi: 10.1042/bj0990682. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BACON J. S., MILNE B. D., TAYLOR I. F., WEBLEY D. M. FEATURES OF THE CELL-WALL STRUCTURE OF YEAST REVEALED BY THE ACTION OF ENZYMES FROM A NON-FRUITING MYXOBACTERIUM (CYTOPHAGA JOHNSONII). Biochem J. 1965 Jun;95:28C–30C. doi: 10.1042/bj0950028c. [DOI] [PubMed] [Google Scholar]
  3. Brown J. P. Susceptibility of the cell walls of some yeasts to lysis by enzymes of Helix pomatia. Can J Microbiol. 1971 Feb;17(2):205–208. doi: 10.1139/m71-035. [DOI] [PubMed] [Google Scholar]
  4. GARCIA MENDOZA C., VILLANUEVA J. R. Production of yeast protoplasts by an enzyme preparation of Streptomyces sp. Nature. 1962 Sep 29;195:1326–1327. doi: 10.1038/1951326a0. [DOI] [PubMed] [Google Scholar]
  5. Jones G. H., Ballou C. E. Isolation of an alpha-mannosidase which hydrolyzes yeast mannan. Structure of the backbone of yeast mannan. J Biol Chem. 1968 May 10;243(9):2442–2446. [PubMed] [Google Scholar]
  6. McLellan W. L., Jr, Lampen J. O. Phosphomannanase (PR-factor), an enzyme required for the formation of yeast protoplasts. J Bacteriol. 1968 Mar;95(3):967–974. doi: 10.1128/jb.95.3.967-974.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Prauser H., Lechevalier M. P., Lechevalier H. Description of Oerskovia gen. n. to harbor Orskov's motile nocardia. Appl Microbiol. 1970 Mar;19(3):534–534. doi: 10.1128/am.19.3.534-534.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. RICHARDSON K. C., JARETT L., FINKE E. H. Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol. 1960 Nov;35:313–323. doi: 10.3109/10520296009114754. [DOI] [PubMed] [Google Scholar]
  10. Sukapure R. S., Lechevalier M. P., Reber H., Higgins M. L., Lechevalier H. A., Prauser H. Motile nocardoid Actinomycetales. Appl Microbiol. 1970 Mar;19(3):527–533. doi: 10.1128/am.19.3.527-533.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. TANAKA H., PHAFF H. J. ENZYMATIC HYDROLYSIS OF YEAST CELL WALLS. I. ISOLATION OF WALL-DECOMPOSING ORGANISMS AND SEPARATION AND PURIFICATION OF LYTIC ENZYMES. J Bacteriol. 1965 Jun;89:1570–1580. doi: 10.1128/jb.89.6.1570-1580.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

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