Abstract
Both reaction-product localization and ferritin-coupled antibody studies have shown that alkaline phosphatase is evenly distributed throughout the peri-plasmic space of Escherichia coli and a marine pseudomonad. This space is not locally enlarged except in cases where plasmolysis has occurred.
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Selected References
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- Brockman R. W., Heppel L. A. On the localization of alkaline phosphatase and cyclic phosphodiesterase in Escherichia coli. Biochemistry. 1968 Jul;7(7):2554–2562. doi: 10.1021/bi00847a016. [DOI] [PubMed] [Google Scholar]
- Cheng K. J., Ingram J. M., Costerton J. W. Alkaline phosphatase localization and spheroplast formation of Pseudomonas aeruginosa. Can J Microbiol. 1970 Dec;16(12):1319–1324. doi: 10.1139/m70-218. [DOI] [PubMed] [Google Scholar]
- Cheng K. J., Ingram J. M., Costerton J. W. Interactions of alkaline phosphatase and the cell wall of Pseudomonas aeruginosa. J Bacteriol. 1971 Jul;107(1):325–336. doi: 10.1128/jb.107.1.325-336.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cheng K. J., Ingram J. M., Costerton J. W. Release of alkaline phosphatase from cells of Pseudomonas aeruginosa by manipulation of cation concentration and of pH. J Bacteriol. 1970 Nov;104(2):748–753. doi: 10.1128/jb.104.2.748-753.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DeVoe I. W., Costerton J. W., MacLeod R. A. Demonstration by freeze-etching of a single cleavage plane in the cell wall of a gram-negative bacterium. J Bacteriol. 1971 May;106(2):659–671. doi: 10.1128/jb.106.2.659-671.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dvorak H. F., Wetzel B. K., Heppel L. A. Biochemical and cytochemical evidence for the polar concentration of periplasmic enzymes in a "minicell" strain of Escherichia coli. J Bacteriol. 1970 Oct;104(1):543–548. doi: 10.1128/jb.104.1.543-548.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heppel L. A. Selective release of enzymes from bacteria. Science. 1967 Jun 16;156(3781):1451–1455. doi: 10.1126/science.156.3781.1451. [DOI] [PubMed] [Google Scholar]
- Knowles C. J. Salt induces changes of turbidity and volume of E. coli. Nat New Biol. 1971 Feb 3;229(5):154–155. doi: 10.1038/newbio229154a0. [DOI] [PubMed] [Google Scholar]
- Kushnarev V. M., Smirnova T. A. Electron microscopy of alkaline phosphatase of Escherichia coli. Can J Microbiol. 1966 Aug;12(4):605–607. doi: 10.1139/m66-086. [DOI] [PubMed] [Google Scholar]
- Nisonson I., Tannenbaum M., Neu H. C. Surface localization of Escherichia coli 5'-nucleotidase by electron microscopy. J Bacteriol. 1969 Nov;100(2):1083–1090. doi: 10.1128/jb.100.2.1083-1090.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schlesinger M. J. Secretion of alkaline phosphatase subunits by spheroplasts of Escherichia coli. J Bacteriol. 1968 Sep;96(3):727–733. doi: 10.1128/jb.96.3.727-733.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thompson J., Costerton J. W., MacLeon R. A. K plus-dependent deplasmolysis of a marine pseudomonad plasmolyzed in a hypotonic solution. J Bacteriol. 1970 Jun;102(3):843–854. doi: 10.1128/jb.102.3.843-854.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wetzel B. K., Spicer S. S., Dvorak H. F., Heppel L. A. Cytochemical localization of certain phosphatases in Escherichia coli. J Bacteriol. 1970 Oct;104(1):529–542. doi: 10.1128/jb.104.1.529-542.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]