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. 1972 Oct;112(1):188–194. doi: 10.1128/jb.112.1.188-194.1972

Evidence for Specific Inhibition of Translocation of Aminoacyl-Transfer Ribonucleic Acid by the Pretreatment of Ribosomes of Bacillus subtilis with p-Chloromercuribenzoate

Rajinder Singh Ranu 1, Akira Kaji 1
PMCID: PMC251395  PMID: 4627920

Abstract

The biological activities of Bacillus subtilis ribosomes pretreated with 0.5, 1, or 2 mmp-chloromercuribenzoate (PCMB) were examined. The ribosomes treated with 1 or 2 mm PCMB lost their capacity to synthesize polyphenylalanine as well as the capacity to bind phenylalanyl-transfer ribonucleic acid. On the other hand, approximately 70% of the polyphenylalanine synthesis capacity was lost with ribosomes pretreated with 0.5 mm PCMB. This inhibition was found to be due to a specific inhibition of the translocation step. The effect of 0.5 mm PCMB was reversed by 20 mm Mg2+ without the loss of PCMB bound to ribosomes, while β-mercaptoethanol partially reversed the inhibitory effect with concomitant loss of bound PCMB.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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