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. 1972 Oct;112(1):206–211. doi: 10.1128/jb.112.1.206-211.1972

Mapping of the fabA Locus for Unsaturated Fatty Acid Biosynthesis in Escherichia coli

John E Cronan Jr 1, David F Silbert 2, Daniel L Wulff 3
PMCID: PMC251398  PMID: 4562395

Abstract

fabA mutants of Escherichia coli require an appropriate unsaturated fatty acid for growth. The fabA locus has now been mapped at minute 21.5 of the linkage map of E. coli. The locus is cotransduced with pyrD and aroA but not with pyrC, purB, or pdxC. The clockwise order of markers in the region is pdxC, aroA, cmlB, pyrD, fabA, pyrC.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cronan J. E., Jr, Birge C. H., Vagelos P. R. Evidence for two genes specifically involved in unsaturated fatty acid biosynthesis in Escherichia coli. J Bacteriol. 1969 Nov;100(2):601–604. doi: 10.1128/jb.100.2.601-604.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Epstein W., Fox C. F. Mapping of a locus for unsaturated fatty acid biosynthesis in Escherichia coli. J Bacteriol. 1970 Jul;103(1):273–274. doi: 10.1128/jb.103.1.273-274.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kass L. R., Bloch K. On the enzymatic synthesis of unsaturated fatty acids in Escherichia coli. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1168–1173. doi: 10.1073/pnas.58.3.1168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Low B. Low recombination frequency for markers very near the origin in conjugation in E. coli. Genet Res. 1965 Nov;6(3):469–473. doi: 10.1017/s0016672300004341. [DOI] [PubMed] [Google Scholar]
  5. MONOD J., COHEN-BAZIRE G., COHN M. Sur la biosynthèse de la beta-galactosidase (lactase) chez Escherichia coli; la spécificité de l'induction. Biochim Biophys Acta. 1951 Nov;7(4):585–599. doi: 10.1016/0006-3002(51)90072-8. [DOI] [PubMed] [Google Scholar]
  6. Pittard J., Wallace B. J. Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli. J Bacteriol. 1966 Apr;91(4):1494–1508. doi: 10.1128/jb.91.4.1494-1508.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Reeve E. C., Doherty P. Linkage relationships of two genes causing partial resistance to chloramphenicol in Escherichia coli. J Bacteriol. 1968 Oct;96(4):1450–1451. doi: 10.1128/jb.96.4.1450-1451.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Schairer H. U., Overath P. Lipids containing trans-unsaturated fatty acids change the temperature characteristic of thiomethylgalactoside accumulation in Escherichia coli. J Mol Biol. 1969 Aug 28;44(1):209–214. doi: 10.1016/0022-2836(69)90416-1. [DOI] [PubMed] [Google Scholar]
  9. Signer E. R., Beckwith J. R., Brenner S. Mapping of suppressor loci in Escherichia coli. J Mol Biol. 1965 Nov;14(1):153–166. doi: 10.1016/s0022-2836(65)80237-6. [DOI] [PubMed] [Google Scholar]
  10. Silbert D. F., Ruch F., Vagelos P. R. Fatty acid replacements in a fatty acid auxotroph of Escherichia coli. J Bacteriol. 1968 May;95(5):1658–1665. doi: 10.1128/jb.95.5.1658-1665.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Silbert D. F., Vagelos P. R. Fatty acid mutant of E. coli lacking a beta-hydroxydecanoyl thioester dehydrase. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1579–1586. doi: 10.1073/pnas.58.4.1579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]

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