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. 1972 Oct;112(1):445–452. doi: 10.1128/jb.112.1.445-452.1972

Reconstitution of Micrococcus lysodeikticus Reduced Nicotinamide Adenine Dinucleotide and l-Malate Dehydrogenases with Dehydrogenase-Depleted Membrane Residues: a Basis for Restoration of Oxidase Actitivies

Robert C Eisenberg 1
PMCID: PMC251431  PMID: 4342817

Abstract

Deoxycholate disruption of Micrococcus lysodeikticus protoplast membranes resulted in solubilization of both l-malate and reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase enzymes (substrate: 2,6-dichlorophenolindophenol oxidoreductases). Insoluble residues contained cytochromes of the b, c, and a type. Solubilized dehydrogenases were reconstituted with insoluble residues by treatment of disrupted membranes with magnesium ions. Most of the solubilized l-malate and NADH dehydrogenase activities were precipitated by magnesium ions independent of enzyme reconstitution with insoluble residues. Reconstituted dehydrogenases explained the mechanism for restoration of disrupted l-malate and NADH oxidase activities (4). Black light irradiation inhibited oxidase activities of both native and reconstituted membranes. These irradiated membrane oxidases were partially restored by exogenous napthoquinones [K2(20) and K2(50)] but not by CoQ(6). Reconstitution experiments showed that native membrane napthoquinone was retained in the insoluble residues of deoxycholate-disrupted membranes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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