Abstract
Extensive stalk elongation in Skl mutants of Caulobacter crescentus occurs when they are grown in complete medium. This stalk elongation is less pronounced in synthetic medium with glucose as the sole carbon source than in complex peptone yeast extract medium. Addition of exogenous nucleoside triphosphates (adenosine triphosphate [ATP], guanosine triphosphate [GTP], cytidine triphosphate, and uridine triphosphate) inhibits stalk elongation of the Skl mutants, whereas cyclic guanosine 3′,5′-monophosphate (GMP) stimulates stalk elongation in the Skl strains grown in synthetic glucose medium. Cyclic GMP also produces stalk elongation in wild-type C. crescentus and concurrently produces a cell division defect resulting in cellular filament formation. Under conditions tested, cyclic adenosine 3′,5′-monophosphate and dibutyryl cyclic adenosine monophosphate did not enhance stalk elongation. Endogenous ATP and GTP levels in the mutants are significantly lower than corresponding nucleotide concentrations of the parent wild-type strains. Control of syntheses resulting in stalk formation in C. crescentus appears to be related to intracellular concentrations of nucleotides, with cyclic GMP as a prominent candidate for an important regulatory role in this aspect of morphogenesis.
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- Agabian-Keshishian N., Shapiro L. Bacterial differentiation and phage infection. Virology. 1971 Apr;44(1):46–53. doi: 10.1016/0042-6822(71)90151-6. [DOI] [PubMed] [Google Scholar]
- Chapman A. G., Fall L., Atkinson D. E. Adenylate energy charge in Escherichia coli during growth and starvation. J Bacteriol. 1971 Dec;108(3):1072–1086. doi: 10.1128/jb.108.3.1072-1086.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Emmer M., deCrombrugghe B., Pastan I., Perlman R. Cyclic AMP receptor protein of E. coli: its role in the synthesis of inducible enzymes. Proc Natl Acad Sci U S A. 1970 Jun;66(2):480–487. doi: 10.1073/pnas.66.2.480. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neuhard J., Randerath E., Randerath K. Ion-exchange thin-layer chromatography. 13. Resolution of complex nucleoside triphosphate mixtures. Anal Biochem. 1965 Nov;13(2):211–222. doi: 10.1016/0003-2697(65)90191-0. [DOI] [PubMed] [Google Scholar]
- Newton A. Role of transcription in the temporal control of development in Caulobacter crescentus (stalk-rifampin-RNA synthesis-DNA synthesis-motility). Proc Natl Acad Sci U S A. 1972 Feb;69(2):447–451. doi: 10.1073/pnas.69.2.447. [DOI] [PMC free article] [PubMed] [Google Scholar]
- POINDEXTER J. S. BIOLOGICAL PROPERTIES AND CLASSIFICATION OF THE CAULOBACTER GROUP. Bacteriol Rev. 1964 Sep;28:231–295. doi: 10.1128/br.28.3.231-295.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pastan I., Perlman R. Cyclic adenosine monophosphate in bacteria. Science. 1970 Jul 24;169(3943):339–344. doi: 10.1126/science.169.3943.339. [DOI] [PubMed] [Google Scholar]
- Riggs A. D., Reiness G., Zubay G. Purification and DNA-binding properties of the catabolite gene activator protein. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1222–1225. doi: 10.1073/pnas.68.6.1222. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmidt J. M., Stanier R. Y. The development of cellular stalks in bacteria. J Cell Biol. 1966 Mar;28(3):423–436. doi: 10.1083/jcb.28.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shapiro L., Agabian-Keshishian N., Bendis I. Bacterial differentiation. Science. 1971 Sep 3;173(4000):884–892. doi: 10.1126/science.173.4000.884. [DOI] [PubMed] [Google Scholar]
- Shapiro L., Agabian-Keshishian N., Hirsch A., Rosen O. M. Effect of dibutyryladenosine 3':5'-cyclic monophosphate on growth and differentiation in Caulobacter crescentus. Proc Natl Acad Sci U S A. 1972 May;69(5):1225–1229. doi: 10.1073/pnas.69.5.1225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zubay G., Schwartz D., Beckwith J. Mechanism of activation of catabolite-sensitive genes: a positive control system. Proc Natl Acad Sci U S A. 1970 May;66(1):104–110. doi: 10.1073/pnas.66.1.104. [DOI] [PMC free article] [PubMed] [Google Scholar]