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. 1972 Nov;112(2):661–665. doi: 10.1128/jb.112.2.661-665.1972

Temperature-Sensitive Osmotic Remedial Mutants of Escherichia coli

R R B Russell 1
PMCID: PMC251472  PMID: 4563969

Abstract

A collection of temperature-sensitive mutants of Escherichia coli K-12 was examined for ability to grow at the restrictive temperature when the osmotic pressure of the medium was increased. Five of the fourteen mutants were found to be osmotic remedial. Four strains containing temperature-sensitive, osmotic-remedial mutations affecting aminoacyl-transfer ribonucleic acid synthetases were found to have altered permeability characteristics which may be attributable to changes in the lipopolysaccharide layer of the cell envelope at restrictive temperatures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. J., Neidhardt F. C. Unusual valyl-transfer ribonucleic acid synthetase mutant of Escherichia coli. J Bacteriol. 1972 Jan;109(1):307–314. doi: 10.1128/jb.109.1.307-314.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BROWN A. D. ASPECTS OF BACTERIAL RESPONSE TO THE IONIC ENVIRONMENT. Bacteriol Rev. 1964 Sep;28:296–329. doi: 10.1128/br.28.3.296-329.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bassel J., Douglas H. C. Relationship between solute permeability and osmotic remediability in a galactose-negative strain of Saccharomyces cerevisiae. J Bacteriol. 1970 Nov;104(2):707–711. doi: 10.1128/jb.104.2.707-711.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Broda P. Ribonucleic acid synthesis and glutamate excretion in Escherichia coli. J Bacteriol. 1968 Nov;96(5):1528–1534. doi: 10.1128/jb.96.5.1528-1534.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CHRISTIAN J. H., WALTHO J. A. THE COMPOSITION OF STAPHYLOCOCCUS AUREUS IN RELATION TO THE WATER ACTIVITY OF THE GROWTH MEDIUM. J Gen Microbiol. 1964 May;35:205–213. doi: 10.1099/00221287-35-2-205. [DOI] [PubMed] [Google Scholar]
  6. Carl P. L. Escherichia coli mutants with temperature-sensitive synthesis of DNA. Mol Gen Genet. 1970;109(2):107–122. doi: 10.1007/BF00269647. [DOI] [PubMed] [Google Scholar]
  7. Foley J. M., Giles N. H., Roberts C. F. Complementation at the adenylosuccinase locus in Aspergillus nidulans. Genetics. 1965 Dec;52(6):1247–1263. doi: 10.1093/genetics/52.6.1247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Good C. M., Pattee P. A. Temperature-Sensitive Osmotically Fragile Mutants of Staphylococcus aureus. J Bacteriol. 1970 Dec;104(3):1401–1403. doi: 10.1128/jb.104.3.1401-1403.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HAWTHORNE D. C., FRIIS J. OSMOTIC-REMEDIAL MUTANTS. A NEW CLASSIFICATION FOR NUTRITIONAL MUTANTS IN YEAST. Genetics. 1964 Nov;50:829–839. doi: 10.1093/genetics/50.5.829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hambleton P. Repair of wall damage in Escherichia coli recovered from an aerosol. J Gen Microbiol. 1971 Nov;69(1):81–88. doi: 10.1099/00221287-69-1-81. [DOI] [PubMed] [Google Scholar]
  11. Knox K. W., Cullen J., Work E. An extracellular lipopolysaccharide-phospholipid-protein complex produced by Escherichia coli grown under lysine-limiting conditions. Biochem J. 1967 Apr;103(1):192–201. doi: 10.1042/bj1030192. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Leive L. Studies on the permeability change produced in coliform bacteria by ethylenediaminetetraacetate. J Biol Chem. 1968 May 10;243(9):2373–2380. [PubMed] [Google Scholar]
  13. Lugtenberg E. J., De Haas-Menger L., Ruyters W. H. Murein synthesis and identification of cell wall precursors of temperature-sensitive lysis mutants of Escherichia coli. J Bacteriol. 1972 Jan;109(1):326–335. doi: 10.1128/jb.109.1.326-335.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mangiarotti G., Apirion D., Schlessinger D. Selection of sucrose-dependent Escherichia coli to obtain envelope mutants and fragile cultures. Science. 1966 Aug 19;153(3738):892–894. doi: 10.1126/science.153.3738.892. [DOI] [PubMed] [Google Scholar]
  15. Matsuzawa H., Matsuhashi M., Oka A., Sugino Y. Genetic and biochemical studies on cell wall peptidoglycan synthesis in Escherichia coli K-12. Biochem Biophys Res Commun. 1969 Aug 15;36(4):682–689. doi: 10.1016/0006-291x(69)90360-x. [DOI] [PubMed] [Google Scholar]
  16. Matzura H., Broda P. Sensitization of Escherichia coli to actinomycin D by the arrest of protein synthesis. J Bacteriol. 1968 Nov;96(5):1877–1879. doi: 10.1128/jb.96.5.1877-1879.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Metzenberg R. L. Repair of multiple defects of a regulatory mutant of Neurospora by high osmotic pressure and by reversion. Arch Biochem Biophys. 1968 May;125(2):532–541. doi: 10.1016/0003-9861(68)90611-5. [DOI] [PubMed] [Google Scholar]
  18. Muschel L. H., Gustafson L. Antibiotic, detergent, and complement sensitivity of Salmonella typhi after ethylenediaminetetraacetic acid treatment. J Bacteriol. 1968 Jun;95(6):2010–2013. doi: 10.1128/jb.95.6.2010-2013.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nashed N., Jabbur G. A genetic and functional characterization of adenine mutants induced in yeast by 1-nitroso-immidazolidone-2 and nitrous acid. Z Vererbungsl. 1966;98(2):106–110. doi: 10.1007/BF00897182. [DOI] [PubMed] [Google Scholar]
  20. Normark S., Westling B. Nature of the penetration barrier in Escherichia coli K-12: effect of macromolecular inhibition of penetrability in strains containing the envA gene. J Bacteriol. 1971 Oct;108(1):45–50. doi: 10.1128/jb.108.1.45-50.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ray B., Jezeski J. J., Busta F. F. Repair of injury in freeze-dried Salmonella anatum. Appl Microbiol. 1971 Sep;22(3):401–407. doi: 10.1128/am.22.3.401-407.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Reeve J. N., Groves D. J., Clark D. J. Regulation of Cell Division in Escherichia coli: Characterization of Temperature-Sensitive Division Mutants. J Bacteriol. 1970 Dec;104(3):1052–1064. doi: 10.1128/jb.104.3.1052-1064.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ricard M., Hirota Y. Effet des sels sur le processus de division cellulaire d'E. coli. C R Acad Sci Hebd Seances Acad Sci D. 1969 Mar 3;268(9):1335–1338. [PubMed] [Google Scholar]
  24. Rothfield L., Pearlman-Kothencz M. Synthesis and assembly of bacterial membrane components. A lipopolysaccharide-phospholipid-protein complex excreted by living bacteria. J Mol Biol. 1969 Sep 28;44(3):477–492. doi: 10.1016/0022-2836(69)90374-x. [DOI] [PubMed] [Google Scholar]
  25. Russell R. R., Pittard A. J. Mutants of Escherichia coli unable to make protein at 42 C. J Bacteriol. 1971 Nov;108(2):790–798. doi: 10.1128/jb.108.2.790-798.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Spratt B. G., Rowbury R. J. A mutant in the initiation of DNA synthesis in Salmonella typhimurium. J Gen Microbiol. 1970 Dec;64(2):127–138. doi: 10.1099/00221287-64-2-127. [DOI] [PubMed] [Google Scholar]
  27. Tamaki S., Sato T., Matsuhashi M. Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12. J Bacteriol. 1971 Mar;105(3):968–975. doi: 10.1128/jb.105.3.968-975.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tempest D. W., Meers J. L., Brown C. M. Influence of environment on the content and composition of microbial free amino acid pools. J Gen Microbiol. 1970 Dec;64(2):171–185. doi: 10.1099/00221287-64-2-171. [DOI] [PubMed] [Google Scholar]

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