Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Nov;112(2):676–681. doi: 10.1128/jb.112.2.676-681.1972

Conditional Lethality of Deletions Which Include uvrB in Strains of Escherichia coli Lacking Deoxyribonucleic Acid Polymerase I

Hiroaki Shizuya a,1, Daniel Dykhuizen a,2
PMCID: PMC251474  PMID: 4563970

Abstract

Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain forming a temperature-sensitive deoxyribonucleic acid polymerase I and carrying a deletion of the uvrB gene is inviable at the nonpermissive temperature.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adhya S., Cleary P., Campbell A. A deletion analysis of prophage lambda and adjacent genetic regions. Proc Natl Acad Sci U S A. 1968 Nov;61(3):956–962. doi: 10.1073/pnas.61.3.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CAMPBELL A. Sensitive mutants of bacteriophage lambda. Virology. 1961 May;14:22–32. doi: 10.1016/0042-6822(61)90128-3. [DOI] [PubMed] [Google Scholar]
  3. Coukell M. B., Yanofsky C. Increased frequency of deletions in DNA polymerase mutants of Escherichia coli. Nature. 1970 Nov 14;228(5272):633–635. doi: 10.1038/228633a0. [DOI] [PubMed] [Google Scholar]
  4. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  5. Gross J. D., Grunstein J., Witkin E. M. Inviability of recA- derivatives of the DNA polymerase mutant of De Lucia and Cairns. J Mol Biol. 1971 Jun 14;58(2):631–634. doi: 10.1016/0022-2836(71)90377-9. [DOI] [PubMed] [Google Scholar]
  6. Gross J., Gross M. Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1166–1168. doi: 10.1038/2241166a0. [DOI] [PubMed] [Google Scholar]
  7. Guarneros G., Echols H. New mutants of bacteriophage lambda with a specific defect in excision from the host chromosome. J Mol Biol. 1970 Feb 14;47(3):565–574. doi: 10.1016/0022-2836(70)90323-2. [DOI] [PubMed] [Google Scholar]
  8. Helling R. B. Recognition of altered deoxyribonucleic acid in recombination. J Bacteriol. 1969 Oct;100(1):224–230. doi: 10.1128/jb.100.1.224-230.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kaiser A. D., Masuda T. Evidence for a prophage excision gene in lambda. J Mol Biol. 1970 Feb 14;47(3):557–564. doi: 10.1016/0022-2836(70)90322-0. [DOI] [PubMed] [Google Scholar]
  10. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  11. Monk M., Kinross J. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I. J Bacteriol. 1972 Mar;109(3):971–978. doi: 10.1128/jb.109.3.971-978.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Monk M., Peacey M., Gross J. D. Repair of damage induced by ultraviolet light in DNA polymerase-defective Escherichia coli cells. J Mol Biol. 1971 Jun 14;58(2):623–630. doi: 10.1016/0022-2836(71)90376-7. [DOI] [PubMed] [Google Scholar]
  13. Neubauer Z., Calef E. Immunity phase-shift in defective lysogens: non-mutational hereditary change of early regulation of lambda prophage. J Mol Biol. 1970 Jul 14;51(1):1–13. doi: 10.1016/0022-2836(70)90265-2. [DOI] [PubMed] [Google Scholar]
  14. Ogawa H., Shimada K., Tomizawa J. Studies on radiation-sensitive mutants of E. coli. I. Mutants defective in the repair synthesis. Mol Gen Genet. 1968 May 3;101(3):227–244. doi: 10.1007/BF00271625. [DOI] [PubMed] [Google Scholar]
  15. Sato K., Campbell A. Specialized transduction of galactose by lambda phage from a deletion lysogen. Virology. 1970 Jul;41(3):474–487. doi: 10.1016/0042-6822(70)90169-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES