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. 1972 Nov;112(2):773–778. doi: 10.1128/jb.112.2.773-778.1972

Location of the Origin of Transfer of the Sex Factor F

N S Willetts 1
PMCID: PMC251485  PMID: 4565418

Abstract

The location of the origin of transfer on the map of Flac has been determined by two different techniques. The first showed that no transfer cistrons were transferred early by a transposition Hfr strain with a known point of chromosomal integration into Flac, and the second used a series of transfer-deficient Hfr deletion strains to map a locus required in cis for chromosome transfer by an incoming Flac factor. Both techniques gave results consistent with a location for the origin of transfer between traJ and the φR11 locus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Achtman M., Willetts N., Clark A. J. Conjugational complementation analysis of transfer-deficient mutants of Flac in Escherichia coli. J Bacteriol. 1972 Jun;110(3):831–842. doi: 10.1128/jb.110.3.831-842.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Broda P., Meacock P., Achtman M. Early transfer of genes determining transfer functions by some Hfr strains in Escherichia coli K12. Mol Gen Genet. 1972;116(4):336–347. doi: 10.1007/BF00270090. [DOI] [PubMed] [Google Scholar]
  4. Clark A. J. The beginning of a genetic analysis of recombination proficiency. J Cell Physiol. 1967 Oct;70(2 Suppl):165–180. doi: 10.1002/jcp.1040700412. [DOI] [PubMed] [Google Scholar]
  5. Clowes R. C., Moody E. E. Chromosomal transfer from "recombination-deficient" strains of Escherichia coli K-12. Genetics. 1966 Apr;53(4):717–726. doi: 10.1093/genetics/53.4.717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cooke M., Meynell E. Chromosomal transfer mediated by de-repressed R factors in F- Escherichia coli K12. Genet Res. 1969 Aug;14(1):79–87. doi: 10.1017/s0016672300001877. [DOI] [PubMed] [Google Scholar]
  7. Freifelder D. Studies on Escherichia coli sex factors. 3. Covalently closed F'Lac DNA molecules. J Mol Biol. 1968 May 28;34(1):31–38. doi: 10.1016/0022-2836(68)90232-5. [DOI] [PubMed] [Google Scholar]
  8. Ippen-Ihler K., Achtman M., Willetts N. Deletion map of the Escherichia coli K-12 sex factor F: the order of eleven transfer cistrons. J Bacteriol. 1972 Jun;110(3):857–863. doi: 10.1128/jb.110.3.857-863.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Manly K. F., Signer E. R., Radding C. M. Nonessential functions of bacteriophage lambda. Virology. 1969 Feb;37(2):177–188. doi: 10.1016/0042-6822(69)90197-4. [DOI] [PubMed] [Google Scholar]
  10. Matsubara K. Properties of sex factor and related episomes isolated from purified Escherichia coli zygote cells. J Mol Biol. 1968 Nov 28;38(1):89–108. doi: 10.1016/0022-2836(68)90130-7. [DOI] [PubMed] [Google Scholar]
  11. Ohki M., Tomizawa J. Asymmetric transfer of DNA strands in bacterial conjugation. Cold Spring Harb Symp Quant Biol. 1968;33:651–658. doi: 10.1101/sqb.1968.033.01.074. [DOI] [PubMed] [Google Scholar]
  12. Otsubo E., Nishimura Y., Hirota Y. Transfer-defective mutants of sex factors in Escherichia coli. I. Defective mutants and complementation analysis. Genetics. 1970 Feb;64(2):173–188. doi: 10.1093/genetics/64.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rupp W. D., Ihler G. Strand selection during bacterial mating. Cold Spring Harb Symp Quant Biol. 1968;33:647–650. doi: 10.1101/sqb.1968.033.01.073. [DOI] [PubMed] [Google Scholar]
  14. STACEY K. A., SIMSON E. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:554–555. doi: 10.1128/jb.90.2.554-555.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Taylor A. L. Current linkage map of Escherichia coli. Bacteriol Rev. 1970 Jun;34(2):155–175. doi: 10.1128/br.34.2.155-175.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Vapnek D., Rupp W. D. Asymmetric segregation of the complementary sex-factor DNA strands during conjugation in Escherichia coli. J Mol Biol. 1970 Nov 14;53(3):287–303. doi: 10.1016/0022-2836(70)90066-5. [DOI] [PubMed] [Google Scholar]
  17. Wilkins B. M. Chromosome transfer from F-lac+ strains of Escherichia coli K-12 mutant at recA, recB, or recC. J Bacteriol. 1969 May;98(2):599–604. doi: 10.1128/jb.98.2.599-604.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Willetts N. S., Finnegan D. J. Characteristics of E. coli K12 strains carrying both an F prime and an R factor. Genet Res. 1970 Aug;16(1):113–122. doi: 10.1017/s0016672300002329. [DOI] [PubMed] [Google Scholar]
  19. Willetts N. S. Plasmid specificity of two proteins required for conjugation in E. coli K12. Nat New Biol. 1971 Apr 7;230(14):183–185. doi: 10.1038/newbio230183a0. [DOI] [PubMed] [Google Scholar]
  20. Willetts N., Achtman M. Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation. J Bacteriol. 1972 Jun;110(3):843–851. doi: 10.1128/jb.110.3.843-851.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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