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. 1972 Nov;112(2):932–939. doi: 10.1128/jb.112.2.932-939.1972

Properties and Purification of N-Acetylmuramyl-l-Alanine Amidase from Staphylococcus aureus H

Howard J Singer 1, Edmund M Wise Jr 1, James T Park 1
PMCID: PMC251505  PMID: 5086664

Abstract

The principal autolytic enzyme activity of the cell sap of Staphylococcus aureus H has been purified 400-fold. It is an N-acetylmuramyl-l-alanine amidase. This enzyme has a molecular weight of 8 to 10 × 105, a pH optimum of 7.3, an ionic strength optimum of 0.16 m and a Km of 10−3m murein repeating units.

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Selected References

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