Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Nov;112(2):1026–1028. doi: 10.1128/jb.112.2.1026-1028.1972

Hyperprotease-Producing Mutants of Bacillus subtilis

Thomas B Higerd 1, James A Hoch 1, John Spizizen 1
PMCID: PMC251520  PMID: 4628743

Abstract

A number of mutants of Bacillus subtilis producing high levels of extracellular protease have been isolated. Analysis of culture supernatants of these mutants has shown that the total amount of proteolytic activity is elevated from 16- to 37-fold over the wild strain. The elevated activity was due to a simultaneous increase in both the neutral and alkaline protease. All of the mutants genetically analyzed were found linked to the argC4 marker by PBS-1 transduction analysis.

Full text

PDF
1026

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balassa G. Biochemical genetics of bacterial sporulation. I. Unidirectional pleiotropic interactions among genes controlling sporulation in Bacillus subtilis. Mol Gen Genet. 1969;104(1):73–103. [PubMed] [Google Scholar]
  2. Boyer H. W., Carlton B. C. Production of two proteolytic enzymes by a transformable strain of Bacillus subtilis. Arch Biochem Biophys. 1968 Nov;128(2):442–455. doi: 10.1016/0003-9861(68)90050-7. [DOI] [PubMed] [Google Scholar]
  3. GUNTELBERG A. V., OTTESEN M. Purification of the proteolytic enzyme from Bacillus subtilis. C R Trav Lab Carlsberg Chim. 1954;29(3-4):36–48. [PubMed] [Google Scholar]
  4. HARDWICK W. A., FOSTER J. W. On the nature of sporogenesis in some aerobic bacteria. J Gen Physiol. 1952 Jul;35(6):907–927. doi: 10.1085/jgp.35.6.907. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hanson R. S., Peterson J. A., Yousten A. A. Unique biochemical events in bacterial sporulation. Annu Rev Microbiol. 1970;24:53–90. doi: 10.1146/annurev.mi.24.100170.000413. [DOI] [PubMed] [Google Scholar]
  6. Hoch J. A. Genetic analysis of pleiotropic negative sporulation mutants in Bacillus subtilis. J Bacteriol. 1971 Mar;105(3):896–901. doi: 10.1128/jb.105.3.896-901.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MCCONN J. D., TSURU D., YASUNOBU K. T. BACILLUS SUBTILIS NEUTRAL PROTEINASE. I. A ZINC ENZYME OF HIGH SPECIFIC ACTIVITY. J Biol Chem. 1964 Nov;239:3706–3715. [PubMed] [Google Scholar]
  8. Millet J. Characterization of proteinases excreted by Bacillus subtilis Marburg strain during sporulation. J Appl Bacteriol. 1970 Mar;33(1):207–219. doi: 10.1111/j.1365-2672.1970.tb05245.x. [DOI] [PubMed] [Google Scholar]
  9. Prestidge L., Gage V., Spizizen J. Protease activities during the course of sporulation on Bacillus subtilis. J Bacteriol. 1971 Sep;107(3):815–823. doi: 10.1128/jb.107.3.815-823.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. RAPPAPORT H. P., RIGGSBY W. S., HOLDEN D. A. A BACILLUS SUBTILIS PROTEINASE. I. PRODUCTION, PURIFICATION, AND CHARACTERIZATION OF A PROTEINASE FROM A TRANSFORMABLE STRAIN OF BACILLUS SUBTILIS. J Biol Chem. 1965 Jan;240:78–86. [PubMed] [Google Scholar]
  11. STEIN H. [Cholecystography in young infants]. Arch Kinderheilkd. 1961 Nov;165:27–33. [PubMed] [Google Scholar]
  12. Schaeffer P. Sporulation and the production of antibiotics, exoenzymes, and exotonins. Bacteriol Rev. 1969 Mar;33(1):48–71. doi: 10.1128/br.33.1.48-71.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shoer R., Rappaport H. P. Analysis of a Bacillus subtilis proteinase mutant. J Bacteriol. 1972 Feb;109(2):575–583. doi: 10.1128/jb.109.2.575-583.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Welker N. E., Campbell L. L. Comparison of the alpha-amylase of Bacillus subtilis and Bacillus amyloliquefaciens. J Bacteriol. 1967 Oct;94(4):1131–1135. doi: 10.1128/jb.94.4.1131-1135.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES