Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1972 Dec;112(3):1169–1176. doi: 10.1128/jb.112.3.1169-1176.1972

Isolation of the rec Mutants in Staphylococcus aureus

Matsuhisa Inoue 1, Hiroshi Oshima 1, Toyoji Okubo 1, Susumu Mitsuhashi 1
PMCID: PMC251545  PMID: 4264448

Abstract

A histidine auxotroph (his−) of Staphylococcus aureus MS3937 and mutants sensitive to ultraviolet (UV) irradiation were obtained. The UV-sensitive mutants were found also to be sensitive to N-methyl-N′-nitro-N-nitrosoguanidine and mitomycin C, and their sensitivity was accounted for by a defect in deoxyribonucleic acid repair. Transduction of either chromosomal or plasmid markers to UV-sensitive mutants showed that these staphylococcus mutants are of the recA (reckless) type mutants as reported in Escherichia coli and Salmonella typhimurium; therefore the UV-sensitive mutants (uvr−) were renamed recombination-deficient mutants (rec−). The biochemical and genetic properties of these mutants are described, and their usefulness for studies of staphylococcal plasmids is discussed.

Full text

PDF
1169

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARBER W. Transduction of chromosomal genes and episomes in Escherichia coli. Virology. 1960 May;11:273–288. doi: 10.1016/0042-6822(60)90066-0. [DOI] [PubMed] [Google Scholar]
  2. Asheshov E. H. Chromosomal location of the genetic elements controlling penicillinase production in a strain of Staphylococcus aureus. Nature. 1966 May 21;210(5038):804–806. doi: 10.1038/210804a0. [DOI] [PubMed] [Google Scholar]
  3. CHABBERT Y. A., BAUDENS J. G., GERBAUD G. R. VARIATIONS SOUS L'INFLUENCE DE L'ACRIFLAVINE ET TRANSDUCTION DE LA R'ESISTANCE A LA KANAMYCINE ET AU CHLORAMPH'ENICOL CHEZ LES STAPHYLOCOQUES. Ann Inst Pasteur (Paris) 1964 Nov;107:678–690. [PubMed] [Google Scholar]
  4. CLARK A. J., MARGULIES A. D. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. doi: 10.1073/pnas.53.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FAIRBROTHER R. W., PARKER L., EATON B. R. The stability of penicillinase-producting strains of Staphylococcus aureus. J Gen Microbiol. 1954 Apr;10(2):309–316. doi: 10.1099/00221287-10-2-309. [DOI] [PubMed] [Google Scholar]
  6. Goering R. V., Pattee P. A. Mutants of Staphylococcus aureus with increased sensitivity to ultraviolet radiation. J Bacteriol. 1971 Apr;106(1):157–161. doi: 10.1128/jb.106.1.157-161.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HASHIMOTO H., KONO K., MITSUHASHI S. ELIMINATION OF PENICILLIN RESISTANCE OF STAPHYLOCOCCUS AUREUS BY TREATMENT WITH ACRIFLAVINE. J Bacteriol. 1964 Jul;88:261–262. doi: 10.1128/jb.88.1.261-262.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hashimoto H., Iyobe S., Mitsuhashi S. Transduction of R factors with phage P1 from recombination-deficient Escherichia coli. Jpn J Microbiol. 1970 Sep;14(5):397–404. doi: 10.1111/j.1348-0421.1970.tb00539.x. [DOI] [PubMed] [Google Scholar]
  9. Hertman I., Luria S. E. Transduction studies on the role of a rec+ gene in the ultraviolet induction of prophage lambda. J Mol Biol. 1967 Jan 28;23(2):117–133. doi: 10.1016/s0022-2836(67)80021-4. [DOI] [PubMed] [Google Scholar]
  10. Howard-Flanders P., Boyce R. P. DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes. Radiat Res. 1966;(Suppl):156+–156+. [PubMed] [Google Scholar]
  11. Inoue M., Hashimoto H., Yamagishi S., Mitsuhashi S. Transduction analysis of the genetic determinants for chloramphenicol resistance in Staphylococci. Jpn J Microbiol. 1970 Jul;14(4):261–268. doi: 10.1111/j.1348-0421.1970.tb00523.x. [DOI] [PubMed] [Google Scholar]
  12. KLOOS W. E., PATTEE P. A. A BIOCHEMICAL CHARACTERIZATION OF HISTIDINE-DEPENDENT MUTANTS OF STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1965 May;39:185–194. doi: 10.1099/00221287-39-2-185. [DOI] [PubMed] [Google Scholar]
  13. Kasuga T., Hashimoto H., Mitsuhashi S. Drug resistance of staphylococci. VII. Genetic determinants responsible for the resistance to tetracycline, streptomycin, sulfanilamide, and penicillin. J Bacteriol. 1968 May;95(5):1764–1766. doi: 10.1128/jb.95.5.1764-1766.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kono M., Ogawa K., Mitsuhashi S. Drug resistance of staphylococci. VI. Genetic determinant for chloramphenicol resistance. J Bacteriol. 1968 Mar;95(3):886–892. doi: 10.1128/jb.95.3.886-892.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MAY J. W., HOUGHTON R. H., PERRET C. J. THE EFFECT OF GROWTH AT ELEVATED TEMPERATURES ON SOME HERITABLE PROPERTIES OF STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1964 Nov;37:157–169. doi: 10.1099/00221287-37-2-157. [DOI] [PubMed] [Google Scholar]
  16. MITSUHASHI S., MORIMURA M., KONO K., OSHIMA H. ELIMINATION OF DRUG RESISTANCE OF STAPHYLOCOCCUS AUREUS BY TREATMENT WITH ACRIFLAVINE. J Bacteriol. 1963 Jul;86:162–164. doi: 10.1128/jb.86.1.162-164.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Morimura M., Watanabe K., Mori H., Mitsuhashi S. Lability of streptomycin resistance in Staphylococcus aureus. Jpn J Microbiol. 1970 Jul;14(4):253–256. doi: 10.1111/j.1348-0421.1970.tb00521.x. [DOI] [PubMed] [Google Scholar]
  18. NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
  19. Novick R. P., Bouanchaud D. The problems of drug-resistant pathogenic bacteria. Extrachromosomal nature of drug resistance in Staphylococcus aureus. Ann N Y Acad Sci. 1971 Jun 11;182:279–294. doi: 10.1111/j.1749-6632.1971.tb30664.x. [DOI] [PubMed] [Google Scholar]
  20. SWANSTROM M., ADAMS M. H. Agar layer method for production of high titer phage stocks. Proc Soc Exp Biol Med. 1951 Nov;78(2):372–375. doi: 10.3181/00379727-78-19076. [DOI] [PubMed] [Google Scholar]
  21. Setlow J. K., Brown D. C., Boling M. E., Mattingly A., Gordon M. P. Repair of deoxyribonucleic acid in Haemophilus influenzae. I. X-ray sensitivity of ultraviolet-sensitive mutants and their behavior as hosts to ultraviolet-irradiated bacteriophage and transforming deoxyribonucleic acid. J Bacteriol. 1968 Feb;95(2):546–558. doi: 10.1128/jb.95.2.546-558.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. VOUREKA A. Induced variations in a penicillin-resistant staphylococcus. J Gen Microbiol. 1952 May;6(3-4):352–360. doi: 10.1099/00221287-6-3-4-352. [DOI] [PubMed] [Google Scholar]
  23. Wing J. P., Levine M., Smith H. O. Recombination-deficient mutant of Salmonella typhimurium. J Bacteriol. 1968 May;95(5):1828–1834. doi: 10.1128/jb.95.5.1828-1834.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES